Mouse monoclonal [MEM-148] to CD18 (Phycoerythrin)
Phycoerythrin. Ex: 488nm, Em: 575nm
This antibody recognizes the epitope exposed on free human CD18 chains
unassociated with CD11 as well as on high-affinity state of LFA-1. We have data to indicate that this antibody may not cross react with Pig. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 20µl reagent/100µl of whole blood or 106 cells in a suspension.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.
Involvement in disease
Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
Belongs to the integrin beta chain family. Contains 1 VWFA domain.
Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.