Anti-CD18 antibody [MEM148] - Low endotoxin, Azide free (ab176540)

Overview

  • Product name
    Anti-CD18 antibody [MEM148] - Low endotoxin, Azide free
    See all CD18 primary antibodies
  • Description
    Mouse monoclonal [MEM148] to CD18 - Low endotoxin, Azide free
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, Functional Studies, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Pig
  • Immunogen

    Tissue, cells or virus corresponding to Human CD18. Peripheral blood mononuclear cells.
    Database link: P05107

  • Positive control
    • Activated myeloid cells.
  • General notes

    Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Properties

Applications

Our Abpromise guarantee covers the use of ab176540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.

Non-reducing conditions.

Functional Studies Use at an assay dependent concentration.

ab176540 induces homotypic cell aggregation and high-affinity LFA-1 conformation in resting leukocytes.

Flow Cyt Use a concentration of 8 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IP Use at an assay dependent concentration.

Target

  • Function
    Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.
  • Involvement in disease
    Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
  • Sequence similarities
    Belongs to the integrin beta chain family.
    Contains 1 VWFA domain.
  • Post-translational
    modifications
    Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 95 subunit beta antibody
    • Beta 2 (antigen CD18 (p95) antibody
    • CD 18 antibody
    • CD18 antibody
    • Cell surface adhesion glycoprotein LFA 1/CR3/P150,959 beta subunit precursor) antibody
    • Cell surface adhesion glycoproteins LFA 1/CR3/p150,95 subunit beta antibody
    • Cell surface adhesion glycoproteins LFA-1/CR3/p150 antibody
    • Complement component 3 receptor 3 and 4 subunit antibody
    • Complement receptor C3 beta subunit antibody
    • Complement receptor C3 subunit beta antibody
    • Integrin beta 2 antibody
    • Integrin beta chain beta 2 antibody
    • Integrin beta-2 (ITGB2) antibody
    • Integrin beta-2 antibody
    • Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) antibody
    • ITB2_HUMAN antibody
    • ITGB2 antibody
    • LAD antibody
    • LCAMB antibody
    • Leukocyte associated antigens CD18/11A, CD18/11B, CD18/11C antibody
    • Leukocyte cell adhesion molecule CD18 antibody
    • LFA 1 antibody
    • LFA1 antibody
    • Lymphocyte function associated antigen 1 (LFA1) antibody
    • Lymphocyte function associated antigen 1 antibody
    • MAC 1 antibody
    • MAC1 antibody
    • Macrophage antigen 1 (mac1) beta antibody
    • MF17 antibody
    • MFI7 antibody
    • OTTHUMP00000115278 antibody
    • OTTHUMP00000115279 antibody
    • OTTHUMP00000115280 antibody
    • OTTHUMP00000115281 antibody
    • OTTHUMP00000115282 antibody
    see all

Images

  • Western Blotting analysis of PMA-activated neutrophils using ab176540. The antibody recognizes the lower 65 to 70 kDa zone (activation marker) as well as the upper 78 to 96 kDa zone (CD18).

  • Western Blotting analysis of PMA-activated monocytes using ab176540. The antibody recognizes the lower 65 to 70 kDa zone (activation marker) as well as the upper 78 to 96 kDa zone (CD18).

References

ab176540 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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