Purified antibody is conjugated with tandem dye PerCP/Cy5.5® under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 4µl for 106 cells.
(or 100 µl of whole blood).
ab157226 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
Involvement in disease
Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.