Overview

  • Product name
    Anti-CD19 antibody [6D5]
    See all CD19 primary antibodies
  • Description
    Rat monoclonal [6D5] to CD19
  • Host species
    Rat
  • Specificity
    CD19 B-cell differentiation antigen
  • Tested applications
    Suitable for: Flow Cyt, IP, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Mouse CD19-expressing K562 human erythroleukemia cells

Properties

Applications

Our Abpromise guarantee covers the use of ab25232 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function
    Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
  • Involvement in disease
    Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
  • Sequence similarities
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications
    Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antibody deficiency due to defect in CD19, included antibody
    • AW495831 antibody
    • B lymphocyte antigen CD19 antibody
    • B lymphocyte surface antigen B4 antibody
    • B-lymphocyte antigen CD19 antibody
    • B-lymphocyte surface antigen B4 antibody
    • B4 antibody
    • CD19 antibody
    • CD19 antigen antibody
    • CD19 molecule antibody
    • Cd19 protein antibody
    • CD19_HUMAN antibody
    • CVID3 antibody
    • Differentiation antigen CD19 antibody
    • Leu 12 antibody
    • Leu-12 antibody
    • Leu12 antibody
    • MGC109570 antibody
    • MGC12802 antibody
    • T-cell surface antigen Leu-12 antibody
    see all

Images

  • Immunohistochemical analysis of renal lymph node with PC3-tumour metastases, staining CD19 with ab25232.

    Cryosections were stored at -80°C and incubated with primary antibodies for 1 hour. After washing with PBS, sections were stained for 1 hour with secondary antibodies.
  • Flow Cytometry analysis of BALB/c mouse splenocyte cells labeling CD19 with ab25232 at 1 μg/106 cells dilution, followed by Rat Anti-Mouse CD3ε-FITC then by Mouse Anti-Rat IgG2a-PE.

References

This product has been referenced in:
  • Yang X  et al. Depletion of microglia augments the dopaminergic neurotoxicity of MPTP. FASEB J 32:3336-3345 (2018). Read more (PubMed: 29401614) »
  • Di Lullo AM  et al. An "ex vivo model" contributing to the diagnosis and evaluation of new drugs in cystic fibrosis. Acta Otorhinolaryngol Ital 37:207-213 (2017). ICC ; Human . Read more (PubMed: 27897275) »
See all 12 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Ileal - Peyer's Patch)
Permeabilization
No
Specification
Ileal - Peyer's Patch
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Formaldehyde

Dr. Jack Robertson

Verified customer

Submitted Oct 04 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Citrate buffer
Permeabilization
No
Specification
Lung
Blocking step
Serum as blocking agent for 1 hour(s) and 5 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Nov 17 2015

Answer

We have one unconjugated antibody ab25232 which unfortunately hasn't been tested in functional assays though it has similar clone number then the tested conjugated ones.

In case you are interested in buying and trying this antibody then I would encourage you to participate in our 100% Abtrial discount programme - a free product can be claimed in exchange of review for this antibody. Please contact me for code if you accept our offer.

More details about the offer can be found at the following link;

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

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Question
Answer

Thank you for contacting us.

Please check the following antibodies;


https://www.abcam.com/cd19-antibody-6d5-pe-cy5-5-ab25548.html

https://www.abcam.com/cd19-antibody-mb19-1-pe-cy5-5-ab25384.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you very much for your interest in ab25232.
To our knowledge, ab25232 has not been tested in IHC-P yet.
We do offer the following antibody that is tested and guaranteed for mouse and IHC-P and might be suitable for your purpose of identifying B cells:
ab64100b (Anti-CD45R antibody) also know as B220
https://www.abcam.com/index.html?datasheet=64100 https://www.abcam.com/index.html?datasheet=64100.
If you prefer to use CD19, I can offer a discount off a future purchase if you buy ab25232 now, test it in IHC-P and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody or the value off your next order.
If you are interested in this offer, please follow these steps:
1. Reply to this e-mail to let me know that you would like to proceed and test ab25232 in IHC-P. I will then send a discount code. This code must be issued before purchasing ab25232 so please wait for my reply before ordering.
2. Purchase ab25232 either by phone, fax, or online (www.abcam.com).
3. Test it in IHC-P.
4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.
5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any product ordered and the discount code is valid for 4 months after issue.
We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab25232 turns out to be unsuitable for IHC-P, you will still receive the discount on your next purchase after your Abreview has been submitted.
Please let me know if you have any questions about this offer and I would be happy to help you further.
The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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