Anti-CD19 antibody [A3-B1] (CF405M) (ab117733)

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Overview

  • Product name
    Anti-CD19 antibody [A3-B1] (CF405M)
    See all CD19 primary antibodies
  • Description
    Mouse monoclonal [A3-B1] to CD19 (CF405M)
  • Host species
    Mouse
  • Conjugation
    CF405M. Ex: 408nm, Em: 452nm
  • Specificity
    Directed against the CD19, which is expressed on human B lymphocytes. ab117733 is B lineage-specific and reacts with early B-cell precursors, pre-pre-B-cells, pre-B- cells, B-cells, intermediate B-cells, mature B-cells and some plasmacytoid cells. Plasma cells were found to be negative. It does not react with other haemopoïetic cells. Also reacts with pre-B-cell- lines, B lymphoblastoid cell-lines and Burkitt cell- lines, and with 50% of myeloma cell-lines. Virtually all non T-ALL, B-CLL and B-cell lymphomas were found to be positive, myeloma cells were found to be negative.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    CD19 antigen which is expressed on human B lymphocytes.

  • Positive control
    • Normal Human peripheral blood
  • General notes

    CD19 cluster expressed from the earliest stages of B-progenitor development and on all peripheral B cells including germinal centre B cells, all B cell lines tested and B cell leukaemias tested. The antigen is lost on B cell maturation to plasma cells.

    Fluorochrome: CF405M (Abs/Em Max: 408/450 nm) CF405M (Abs/Em Max: 408/450nm). Direct replacement for Pacific Blue dye®, BD Horizon™ V450

Properties

Applications

Our Abpromise guarantee covers the use of ab117733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 5µl for 106 cells.

ab126036 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
  • Involvement in disease
    Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
  • Sequence similarities
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications
    Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • Antibody deficiency due to defect in CD19, included antibody
      • AW495831 antibody
      • B lymphocyte antigen CD19 antibody
      • B lymphocyte surface antigen B4 antibody
      • B-lymphocyte antigen CD19 antibody
      • B-lymphocyte surface antigen B4 antibody
      • B4 antibody
      • CD19 antibody
      • CD19 antigen antibody
      • CD19 molecule antibody
      • Cd19 protein antibody
      • CD19_HUMAN antibody
      • CVID3 antibody
      • Differentiation antigen CD19 antibody
      • Leu 12 antibody
      • Leu-12 antibody
      • Leu12 antibody
      • MGC109570 antibody
      • MGC12802 antibody
      • T-cell surface antigen Leu-12 antibody
      see all

    Images

    • Flow Cytometry - Anti-CD19 antibody [A3-B1] (CF405M) (ab117733)
      Flow Cytometry - Anti-CD19 antibody [A3-B1] (CF405M) (ab117733)
      Staining of CD19 using Normal Human peripheral blood with ab117733 (5 µl/106 cells).
    • Flow Cytometry - Anti-CD19 antibody [A3-B1] (CF405M) (ab117733)
      Flow Cytometry - Anti-CD19 antibody [A3-B1] (CF405M) (ab117733)
      Staining of CD19 using Normal Human peripheral blood with ab117733 (5 µl/106 cells).

    References

    ab117733 has not yet been referenced specifically in any publications.

    Publishing research using ab117733? Please let us know so that we can cite the reference in this datasheet.

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