• Product name

  • Description

    Rabbit polyclonal to CD19
  • Host species

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human CD19 (internal sequence). The exact sequence is proprietary.
    Database link: P15391

  • Positive control

    • Flow Cyt: Mouse splenocytes. WB: Mouse leukocyte extract; Raji whole cell lysate. ICC/IF: Raji cells.



Our Abpromise guarantee covers the use of ab227019 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 61 kDa.
ICC/IF 1/100 - 1/1000.
Flow Cyt 1/50 - 1/200.


  • Function

    Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
  • Involvement in disease

    Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
  • Sequence similarities

    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational

    Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • Antibody deficiency due to defect in CD19 antibody
    • Antibody deficiency due to defect in CD19, included antibody
    • AW495831 antibody
    • B lymphocyte antigen CD19 antibody
    • B lymphocyte surface antigen B4 antibody
    • B-lymphocyte antigen CD19 antibody
    • B-lymphocyte surface antigen B4 antibody
    • B4 antibody
    • CD19 antibody
    • CD19 antigen antibody
    • CD19 molecule antibody
    • Cd19 protein antibody
    • CD19_HUMAN antibody
    • CVID3 antibody
    • Differentiation antigen CD19 antibody
    • Leu 12 antibody
    • Leu-12 antibody
    • Leu12 antibody
    • MGC109570 antibody
    • MGC12802 antibody
    • T-cell surface antigen Leu-12 antibody
    see all


  • Flow cytometric analysis of 4% paraformaldehyde-fixed mouse splenocytes labeling CD19 with ab227019 at 1/50 dilution (red) compared with an unlabeled sample as a control (black).

    Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

  • Anti-CD19 antibody (ab227019) at 1/1000 dilution + Mouse leukocyte extract at 50 µg

    Predicted band size: 61 kDa

    7.5% SDS-PAGE gel.

  • Raji (human Burkitt's lymphoma cell line) cells stained for CD19 (green) using ab227019 at 1/1000 dilution in ICC/IF. Cells were fixed in ice-cold methanol for 5 minutes.

    Blue: Hoechst 33342 staining.

  • Anti-CD19 antibody (ab227019) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg

    Predicted band size: 61 kDa

    7.5 % SDS-PAGE gel.


ab227019 has not yet been referenced specifically in any publications.

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