PE Anti-CD19 antibody [CB19] (ab1168)
Key features and details
- PE Mouse monoclonal [CB19] to CD19
- Suitable for: Flow Cyt
- Reacts with: Human, Chimpanzee, Macaque monkey, Rhesus monkey
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
PE Anti-CD19 antibody [CB19]
See all CD19 primary antibodies -
Description
PE Mouse monoclonal [CB19] to CD19 -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human, Chimpanzee, Macaque monkey, Rhesus monkey -
Immunogen
Tissue, cells or virus. Intact normal human B cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
CB19 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1168 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt | (1) |
Use at an assay dependent concentration.
Characterization of leukemias and lymphomas in human lysed whole peripheral blood or mononuclear cells separated by density gradient. CD19 (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers. 10ul of CD19 (PE) is sufficient for labelling of 1x10^6 cells. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
Use at an assay dependent concentration. Characterization of leukemias and lymphomas in human lysed whole peripheral blood or mononuclear cells separated by density gradient. CD19 (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers. 10ul of CD19 (PE) is sufficient for labelling of 1x10^6 cells. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. -
Involvement in disease
Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsPhosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 930 Human
- Entrez Gene: 705211 Rhesus monkey
- Omim: 107265 Human
- SwissProt: P15391 Human
- Unigene: 652262 Human
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Alternative names
- Antibody deficiency due to defect in CD19 antibody
- Antibody deficiency due to defect in CD19, included antibody
- AW495831 antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab1168 has been referenced in 4 publications.
- Kushnir A et al. Ryanodine Receptor Calcium Leak in Circulating B-Lymphocytes as a Biomarker in Heart Failure. Circulation 138:1144-1154 (2018). PubMed: 29593014
- Pyöriä L et al. Extinct type of human parvovirus B19 persists in tonsillar B cells. Nat Commun 8:14930 (2017). Flow Cyt . PubMed: 28374737
- Bradbury LE et al. The CD19/CD21 signal transducing complex of human B lymphocytes includes the target of antiproliferative antibody-1 and Leu-13 molecules. J Immunol 149:2841-50 (1992). PubMed: 1383329
- Carter RH & Fearon DT CD19: lowering the threshold for antigen receptor stimulation of B lymphocytes. Science 256:105-7 (1992). PubMed: 1373518