Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (palatine tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
palatine tonsil
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Other product details
Incubation time
19 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Normal Goat Serum
Secondary antibody
Name
Non-Abcam antibody was used: Dako EnVision+ System- HRP Labelled Polymer Anti-R
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Additional data
Abcam response
We would like to thank the abreviewer for the effort in testing this antibody in IHC-P with Human samples. As we have not tested this antibody with human samples on this technique we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Jan 11 2021