Anti-CD19 antibody [LT19], prediluted (Phycoerythrin) (ab18229)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-CD19 antibody [LT19], prediluted (Phycoerythrin)
See all CD19 primary antibodies -
DescriptionMouse monoclonal [LT19] to CD19, prediluted (Phycoerythrin)
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Host speciesMouse
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ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
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Tested applicationsSuitable for: Flow Cytmore details
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Species reactivityReacts with: Human
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Immunogen
Human B lymphoblast cell line Daudi.
Properties
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FormPrediluted
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituents: PBS, BSA -
Concentration information loading... -
PurityProtein G purified
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Purification notesConjugate purified by size-exclusion chromatography
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ClonalityMonoclonal
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Clone numberLT19
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IsotypeIgG1
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab18229 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. 20 µl for a 100 µl sample is recommended.
ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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FunctionAssembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
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Involvement in diseaseDefects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
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Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
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Post-translational
modificationsPhosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 930 Human
- Omim: 107265 Human
- SwissProt: P15391 Human
- Unigene: 652262 Human
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Alternative names
- Antibody deficiency due to defect in CD19, included antibody
- AW495831 antibody
- B lymphocyte antigen CD19 antibody
see all
Images
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![Flow Cytometry - Anti-CD19 antibody [LT19], prediluted (Phycoerythrin) (ab18229)](https://www.abcam.com/ps/products/18/ab18229/Images/ab18229-1-Ab18229FC.jpg)
Flow Cytometry - Anti-CD19 antibody [LT19], prediluted (Phycoerythrin) (ab18229)Surface staining of human peripheral blood cells with anti-human CD19 (LT19) PE.�
Protocols
References
This product has been referenced in:
- te Vruchte D et al. Relative acidic compartment volume as a lysosomal storage disorder-associated biomarker. J Clin Invest 124:1320-8 (2014). Flow Cyt ; Human . Read more (PubMed: 24487591) »
