Recombinant
RabMAb

Recombinant Anti-CD19 antibody [SP110] - BSA and Azide free (ab240402)

Overview

  • Product name

    Anti-CD19 antibody [SP110] - BSA and Azide free
    See all CD19 primary antibodies
  • Description

    Rabbit monoclonal [SP110] to CD19 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide within Human CD19 aa 500 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P15391

    Synthetic peptide within Human CD19 aa 500 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P15391

  • Positive control

    • IHC-P: Human tonsil tissue FC: Raji cells
  • General notes

    Ab240402 is the carrier-free version of ab225717. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240402 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Applications

Our Abpromise guarantee covers the use of ab240402 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol. Primary incubation for 30 minutes at room temperature. 

 

Flow Cyt Use at an assay dependent concentration.

Primary antibody incubation for 30 minutes at at 4°C.

Target

  • Function

    Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
  • Involvement in disease

    Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
  • Sequence similarities

    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications

    Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Antibody deficiency due to defect in CD19 antibody
    • Antibody deficiency due to defect in CD19, included antibody
    • AW495831 antibody
    • B lymphocyte antigen CD19 antibody
    • B lymphocyte surface antigen B4 antibody
    • B-lymphocyte antigen CD19 antibody
    • B-lymphocyte surface antigen B4 antibody
    • B4 antibody
    • CD19 antibody
    • CD19 antigen antibody
    • CD19 molecule antibody
    • Cd19 protein antibody
    • CD19_HUMAN antibody
    • CVID3 antibody
    • Differentiation antigen CD19 antibody
    • Leu 12 antibody
    • Leu-12 antibody
    • Leu12 antibody
    • MGC109570 antibody
    • MGC12802 antibody
    • T-cell surface antigen Leu-12 antibody
    see all

Images

  • Flow cytometry analysis of Raji (human Burkitt's lymphoma) labeling CD19 with purified ab225717 at 1/40 dilution (11.725 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225717).

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD19 using ab225717 at 1/200 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab225717).

  • Flow cytometric analysis of Ramos (human Burkitt's lymphoma cell line) cell line labeling CD19 with ab225717 at 1/100 dilution (green) compared with a negative control of rabbit IgG (blue)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225717).

References

ab240402 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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