Recombinant Anti-CD19 antibody [SP291] - BSA and Azide free (ab237772)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP291] to CD19 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), IHC-P, WB, mIHC
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD19 antibody [SP291] - BSA and Azide free
See all CD19 primary antibodies -
Description
Rabbit monoclonal [SP291] to CD19 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, WB, mIHCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt (intra): Ramos and Raji cells. IHC-P: Human tonsil, spleen, colon, thymus, B-Cell lymphoma and breast ductal carcinoma tissues. mIHC: Human colon, liver, tonsil tissues.
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General notes
ab237772 is the carrier-free version of ab227688.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP291 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-CD19 antibody [SP291] - C-terminal (ab227688)
- PE Anti-CD19 antibody [SP291] (C-terminal) (ab305997)
- APC Anti-CD19 antibody [SP291] (C-terminal) (ab305998)
- HRP Anti-CD19 antibody [SP291] (C-terminal) (ab305999)
- Alexa Fluor® 488 Anti-CD19 antibody [SP291] - C-terminal (ab309942)
- Alexa Fluor® 647 Anti-CD19 antibody [SP291] - C-terminal (ab310309)
- Alexa Fluor® 594 Anti-CD19 antibody [SP291] - C-terminal (ab310773)
- Alexa Fluor® 555 Anti-CD19 antibody [SP291] - C-terminal (ab312299)
- Alexa Fluor® 568 Anti-CD19 antibody [SP291] - C-terminal (ab312795)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab237772 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol. |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 61 kDa.
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|
mIHC |
1/5000.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) |
Notes |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 61 kDa. |
mIHC
1/5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) |
Target
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Function
Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. -
Involvement in disease
Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsPhosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 930 Human
- Omim: 107265 Human
- SwissProt: P15391 Human
- Unigene: 652262 Human
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Alternative names
- Antibody deficiency due to defect in CD19 antibody
- Antibody deficiency due to defect in CD19, included antibody
- AW495831 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver cancer labelling CD208 with ab281573 at 1/1200 (B), CD3E with ab237707 at 1/1200 dilution (C) and CD19 with ab237772 at 1/1200 dilution(D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Panel A: merged staining of anti-CD208 (red; Opal™570), anti-CD3E (green; Opal™520) and anti-CD19 (magenta; Opal™690) on human liver cancer.
Panel B: anti-CD208 staining dendritic cells in human liver cancer.
Panel C: anti-CD3E staining T lymphocytes in human liver cancer.
Panel D: anti-CD19 staining B lymphocytes of human liver cancer.The section was incubated in three rounds of staining: in the order ab281573, ab237707, and ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon cancer labelling CD208 with ab281573 at 1/1200 (B), CD3E with ab237707 at 1/1200 dilution (C) and CD19 with ab237772 at 1/1200 dilution(D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Panel A: merged staining of anti-CD208 (red; Opal™570), anti-CD3E (green; Opal™520) and anti-CD19 (magenta; Opal™690) on human colon cancer.
Panel B: anti-CD208 staining dendritic cells in human colon cancer.
Panel C: anti-CD3E staining T lymphocytes in human colon cancer.
Panel D: anti-CD19 staining B lymphocytes of human colon cancer.The section was incubated in three rounds of staining: in the order ab281573, ab237707, and ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon labelling CD208 with ab281573 at 1/1200 (B), CD3E with ab237707 at 1/1200 dilution (C) and CD19 with ab237772 at 1/1200 dilution(D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Panel A: merged staining of anti-CD208 (red; Opal™570), anti-CD3E (green; Opal™520) and anti-CD19 (magenta; Opal™690) on human colon.
Panel B: anti-CD208 staining dendritic cells in human colon.
Panel C: anti-CD3E staining T lymphocytes in human colon.
Panel D: anti-CD19 staining B lymphocytes of human colon.The section was incubated in three rounds of staining: in the order ab281573, ab237707, and ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil labelling CD208 with ab281573 at 1/1200 (B), CD3E with ab237707 at 1/1200 dilution (C) and CD19 with ab237772 at 1/1200 dilution(D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Panel A: merged staining of anti-CD208 (red; Opal™570), anti-CD3E (green; Opal™520) and anti-CD19 (magenta; Opal™690) on human tonsil.
Panel B: anti-CD208 staining dendritic cells in human tonsil.
Panel C: anti-CD3E staining T lymphocytes in human tonsil.
Panel D: anti-CD19 staining B lymphocytes of human tonsil.The section was incubated in three rounds of staining: in the order ab281573, ab237707, and ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
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This image was generated using ab227688, the same clone, but with a different buffer formulation.
Panel A: merged staining of anti-CD68 (gray; Opal™690), anti-CD3 (green; Opal™520) and anti-CD19 (red; Opal™570) on Formalin/PFA-fixed paraffin-embedded sections of human tonsil. Secondary antibody was Opal Polymer HRP Ms + Rb, and couterstaining was with DAPI.
Panel B: anti-CD3 stained on T cells with ab16669 at 1/500 dilution
Panel C: anti-CD19 stained on B cells with ab237772 at 1/5000 dilution
Panel D: anti-CD68 stained on macrophages with ab213363 1/500 dilutionThe section was incubated in three rounds of staining: in the order of ab213363 and ab16669 for 30 mins, then ab237772 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
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This image was generated using ab227688, the same clone, but with a different buffer formulation.
Panel A: merged staining of anti-CD68 (magenta; Opal™690), anti-CD3 (green; Opal™520) and anti-CD19 (red; Opal™570) on Formalin/PFA-fixed paraffin-embedded sections of human spleen. Secondary antibody was Opal Polymer HRP Ms + Rb, and counterstaining was with DAPI.
Panel B: anti-CD3 stained on T cells with ab16669 at 1/500 dilution
Panel C: anti-CD19 stained on B cells with ab237772 at 1/5000 dilution
Panel D: anti-CD68 stained on macrophages with ab213363 1/500 dilutionThe section was incubated in three rounds of staining: in the order of ab213363 and ab16669 for 30 mins, then ab237772 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
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This image was generated using ab227688, the same clone, but with a different buffer formulation.
Panel A: merged staining of anti-CD31 (gray; Opal™690), anti-CD3 (green; Opal™520) and anti-CD19 (red; Opal™570) on Formalin/PFA-fixed paraffin-embedded sections of human tonsil. Secondary antibody was Opal Polymer HRP Ms + Rb, nuclear counterstain was DAPI.
Panel B: anti-CD3 stained on T cells with ab16669 at 1/500 dilution
Panel C: anti-CD19 stained on B cells with ab237772 at 1/5000 dilution
Panel D: anti-CD31 stained on endothelial cells and immune cell subsets with ab207090 at 1/500 dilution
The section was incubated in three rounds of staining: in the order of ab207090 and ab16669 for 30 mins, then ab237772 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins -
This image was generated using ab227688, the same clone, but with a different buffer formulation.
Panel A: merged staining of anti-CD31 (gray; Opal™690), anti-CD3 (green; Opal™520) and anti-CD19 (red; Opal™570) on Formalin/PFA-fixed paraffin-embedded sections of human spleen. Secondary antibody was Opal Polymer HRP Ms + Rb, nuclear counterstain was DAPI.
Panel B: anti-CD3 stained on T cells with ab16669 at 1/500 dilution
Panel C: anti-CD19 stained on B cells with ab237772 at 1/5000 dilution
Panel D: anti-CD31 stained on endothelial cells with ab207090 at 1/500 dilution
The section was incubated in three rounds of staining: in the order of ab207090 and ab16669 for 30 mins, then ab237772 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD19 with ab227688 at 1/100 dilution (1.50 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Membranous staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227688 for 10 mins at room temperature. This image was generated using ab227688, the same clone, but with a different buffer formulation. -
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human tonsil tissue labeling CD8 alpha with ab245118 at 1/500 dilution, CD4 with ab238798 at 1/500, and CD19 with ab237772 at 1/5000 dilution.
Panel A: merged staining of anti-CD8 alpha (magenta; Opal™690), anti-CD4 (green; Opal™520) and anti-CD19 (red; Opal™570) on human tonsil.
Panel B: anti-CD8 alpha stained on cytotoxic T cells.
Panel C: anti-CD4 stained on T helper cells.
Panel D: anti-CD19 stained on B cells.
Sections were treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins before antibody incubation. The section was incubated in three rounds of staining: in the order of ab245118 for 30 mins, then ab238798 and ab237772 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.DAPI was used as a nuclear counterstain.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. -
Intracellular Flow Cytometry analysis of Raji (human Burkitt's lymphoma) labeling CD19 with purified ab227688 at 1/20 dilution (7.5µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227688).
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Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human tonsil tissue labeling CD8 alpha with ab245118 at 1/500 dilution, CD4 with ab238798 at 1/500, and CD19 with ab237772 at 1/5000 dilution.
Panel A: merged staining of anti-CD8 alpha (magenta; Opal™690), anti-CD4 (green; Opal™520) and anti-CD19 (red; Opal™570) on human tonsil.
Panel B: anti-CD8 alpha stained on cytotoxic T cells.
Panel C: anti-CD4 stained on T helper cells.
Panel D: anti-CD19 stained on B cells.
Sections were treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins before antibody incubation. The section was incubated in three rounds of staining: in the order of ab245118 for 30 mins, then ab238798 and ab237772 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.DAPI was used as a nuclear counterstain.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. -
Intracellular Flow Cytometry analysis of Ramos (human Burkitt's lymphoma cell line) cells, labeling CD19 with ab227688 at 1/400 dilution (green) compared to a Rabbit IgG negative control (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227688).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab237772 has not yet been referenced specifically in any publications.