Anti-CD19 (phospho Y531) antibody (ab203615)
Key features and details
- Rabbit polyclonal to CD19 (phospho Y531)
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CD19 (phospho Y531) antibody
See all CD19 primary antibodies -
Description
Rabbit polyclonal to CD19 (phospho Y531) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide within Human CD19 aa 500 to the C-terminus (phospho Y531) conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P15391 -
Positive control
- Human esophageal cancer tissue; Mouse fetal liver and spleen tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203615 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | (1) |
1/100 - 1/500.
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Notes |
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IHC-P
1/100 - 1/500. |
Target
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Function
Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. -
Involvement in disease
Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsPhosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 930 Human
- Entrez Gene: 12478 Mouse
- Entrez Gene: 365367 Rat
- Omim: 107265 Human
- SwissProt: P15391 Human
- SwissProt: P25918 Mouse
- Unigene: 652262 Human
- Unigene: 4360 Mouse
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Alternative names
- Antibody deficiency due to defect in CD19 antibody
- Antibody deficiency due to defect in CD19, included antibody
- AW495831 antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human esophageal cancer tissue labeling CD19 (phospho Y531) with ab203615 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Immunohistochemical analysis of paraformaldehyde-fixed, paraffin-embedded mouse fetal liver tissue labeling CD19 (phospho Y531) with ab203615 at 1/500 dilution, followed by conjugation to the secondary antibody and DAB staining.
Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min.
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Immunohistochemical analysis of paraformaldehyde-fixed, paraffin-embedded mouse spleen tissue labeling CD19 (phospho Y531) with ab203615 at 1/500 dilution, followed by conjugation to the secondary antibody and DAB staining.
Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min.
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Immunohistochemical analysis of paraformaldehyde-fixed, paraffin-embedded mouse spleen tissue labeling CD19 (phospho Y531) with ab203615 at 1/500 dilution, followed by conjugation to the secondary antibody and DAB staining.
Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min.
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab203615 has been referenced in 11 publications.
- Bai X et al. Adaptive antitumor immune response stimulated by bio-nanoparticle based vaccine and checkpoint blockade. J Exp Clin Cancer Res 41:132 (2022). PubMed: 35392977
- Zhu Y et al. Involvement of MST1/mTORC1/STAT1 activity in the regulation of B-cell receptor signalling by chemokine receptor 2. Clin Transl Med 12:e887 (2022). PubMed: 35875970
- Du Z et al. STAT3 couples with 14-3-3s to regulate BCR signaling, B-cell differentiation, and IgE production. J Allergy Clin Immunol 147:1907-1923.e6 (2021). PubMed: 33045280
- He X et al. Tumor-initiating stem cell shapes its microenvironment into an immunosuppressive barrier and pro-tumorigenic niche. Cell Rep 36:109674 (2021). PubMed: 34496236
- Yang L et al. CCL2 regulation of MST1-mTOR-STAT1 signaling axis controls BCR signaling and B-cell differentiation. Cell Death Differ 28:2616-2633 (2021). PubMed: 33879857