Product nameAnti-CD1a antibody [EP3091]
See all CD1a primary antibodies
DescriptionRabbit monoclonal [EP3091] to CD1a
Tested applicationsSuitable for: WB, ICCmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human CD1a aa 200-300. The exact sequence is proprietary.
- MOLT4, K562 and fetal thymus lysates
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab76531 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 37 kDa.|
|ICC||1/100 - 1/250.|
FunctionAntigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.
Tissue specificityExpressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell leukemias, and in various other tissues.
Sequence similaritiesContains 1 Ig-like (immunoglobulin-like) domain.
Cellular localizationCell membrane. Endosome membrane. Subject to intracellular trafficking between the cell membrane and endosomes. Localizes to cell surface lipid rafts.
- Information by UniProt
- CD 1a antibody
- CD1 antibody
- CD1a antibody
All lanes : Anti-CD1a antibody [EP3091] (ab76531) at 1/5000 dilution
Lane 1 : MOLT4 lysate
Lane 2 : K562 lysate
Lane 3 : Fetal thymus lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 37 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?
ab76531 has been referenced in 1 publication.
- Mahiddine K et al. CD99 isoforms regulate CD1a expression in human monocyte-derived DCs through ATF-2/CREB-1 phosphorylation. Eur J Immunol 46:1460-71 (2016). PubMed: 27094031