Recombinant
RabMAb

Recombinant Anti-CD1a antibody [EP3622] (ab108309)

Overview

  • Product name

    Anti-CD1a antibody [EP3622]
    See all CD1a primary antibodies
  • Description

    Rabbit monoclonal [EP3622] to CD1a
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in human CD1a

  • Positive control

    • Human fetal thymus lysate, human T-78 cell lysate, human skin tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.30 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP3622
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.
    • Tissue specificity

      Expressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell leukemias, and in various other tissues.
    • Sequence similarities

      Contains 1 Ig-like (immunoglobulin-like) domain.
    • Cellular localization

      Cell membrane. Endosome membrane. Subject to intracellular trafficking between the cell membrane and endosomes. Localizes to cell surface lipid rafts.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD 1a antibody
      • CD1 antibody
      • CD1a antibody
      • CD1A Antigen antibody
      • CD1A antigen, a polypeptide antibody
      • CD1a molecule antibody
      • CD1A_HUMAN antibody
      • cluster of differentiation 1 A antibody
      • cortical thymocyte antigen CD1A antibody
      • differentiation antigen CD1 alpha 3 antibody
      • epidermal dendritic cell marker CD1a antibody
      • FCB 6 antibody
      • FCB6 antibody
      • HTA 1 antibody
      • HTA1 antibody
      • hTa1 thymocyte antigen antibody
      • OTTHUMP00000018907 antibody
      • R 4 antibody
      • R4 antibody
      • T 6 antibody
      • T-cell surface antigen T6/Leu-6 antibody
      • T-cell surface glycoprotein CD1a antibody
      • T6 antibody
      see all

    Images

    • ab108309, at 1/250, staining CD1a in human skin tissue by immunohistochemistry

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab108309 showing positive staining in Normal tonsil tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • All lanes : Anti-CD1a antibody [EP3622] (ab108309) at 1/1000 dilution

      Lane 1 : Human fetal thymus lysate
      Lane 2 : T-78 cell lysate at 10 µg

      Performed under reducing conditions.

      Predicted band size: 37 kDa

    • ab108309 showing negative staining in Normal stomach tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab108309 showing negative staining in Normal uterus tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab108309 showing negative staining in Normal breast tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab108309 showing negative staining in Normal kidney tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Bolognesi MM  et al. Multiplex Staining by Sequential Immunostaining and Antibody Removal on Routine Tissue Sections. J Histochem Cytochem 65:431-444 (2017). Read more (PubMed: 28692376) »
    • Nguyen N  et al. Tumor infiltrating lymphocytes and survival in patients with head and neck squamous cell carcinoma. Head Neck 38:1074-84 (2016). Human . Read more (PubMed: 26879675) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you very much for your phone call.

    The best antibodies we can recommend are as follows. Please note that the recommendations are based on immunogen sequence only we currently do not hold any rabbit specific data.

    Anti CD3 : ab16669; I can provide Abreview image discount code and will also provide Abpromise guarantee on use for this ab.

    Anti FOXP3: ab20034, ab22510 are best antibodies we can offer. Eligible for new species Abreview discount code however not eligible for refund in case it does not work.

    Anti CD4: ab61131, ab101530 and ab108329, are the best antibodies we can offer. Eligible for new species Abreview discount code however not eligible for refund in case it does not work.

    anti CD8: ab108343, ab123630 and ab86733, are the best antibodies we can offer. Eligible for new species Abreview discount code however not eligible for refund in case it does not work.

    Anti CD1A : ab76531, ab108309 and ab123133 are the best antibodies we can offer. Eligible for new species Abreview discount code however not eligible for refund in case it does not work.

    Please also note that most of these antibodies are raised in Rabbit so there will be high back ground staining. Our recommendations to reduce the back ground staining are

    - Use preadsorbed anti body e.g. ab7132, ab7083, ab

    - Use (Fab)2 fragments antibodies e.g. ab6112, ab102296

    - Incubate sections with affinity pure Fab fragment anti rabbit antibody before applying primary antibody

    Please check the full details by clicking the links;

    https://www.abcam.com/index.html?pageconfig=resource&rid=12722
    https://www.abcam.com/index.html?pageconfig=resource&rid=12825
    https://www.abcam.com/index.html?pageconfig=resource&rid=11465

    The abreview discount code details can be found by clicking following link;

    https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

    Could you please select the antibodies you are interested in, I will then be happy in creating a discount code for each one of these.

    I will look forward to hearing from you soon.

    Read More

    Answer

    Thank you for contacting us. The immunogen of ab108309 is from amino acid XXXXXXXX of human CD1a protein. The immunogen of ab76531 is from amino acid XXXXXX of human CD1a. Click to check the compatible products: https://www.abcam.com/index.html?pageconfig=productmap&cl=918 https://www.abcam.com/index.html?pageconfig=resource&rid=13148 https://www.abcam.com/index.html?pageconfig=resource&rid=12906 I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

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