Recombinant Anti-CD1a antibody [EP3622] (ab108309)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP3622] to CD1a
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD1a antibody [EP3622]
See all CD1a primary antibodies -
Description
Rabbit monoclonal [EP3622] to CD1a -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal thymus lysate, human T-78 cell lysate, human skin tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.30 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP3622 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108309 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For unpurified use at 1/250 dilution. |
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa. |
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For unpurified use at 1/250 dilution. |
Target
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Function
Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. -
Tissue specificity
Expressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell leukemias, and in various other tissues. -
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. Endosome membrane. Subject to intracellular trafficking between the cell membrane and endosomes. Localizes to cell surface lipid rafts. - Information by UniProt
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Database links
- Entrez Gene: 909 Human
- Omim: 188370 Human
- SwissProt: P06126 Human
- Unigene: 1309 Human
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Alternative names
- CD 1a antibody
- CD1 antibody
- CD1a antibody
see all
Images
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All lanes : Anti-CD1a antibody [EP3622] (ab108309) at 1/1000 dilution
Lane 1 : Wild-type Jurkat cell lysate
Lane 2 : CD1A knockout Jurkat cell lysate
Lane 3 : MOLT-4 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 45-50 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-CD1a antibody [EP3622] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab108309 was shown to bind specifically to CD1a. A band was observed at 45-50 kDa in wild-type Jurkat cell lysates with no signal observed at this size in CD1A knockout cell line ab274926 (knockout cell lysate ab274984). To generate this image, wild-type and CD1A knockout Jurkat cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thymoma tissue sections labelling CD1a with purified ab108309 at 1/1000 dilution (0.14 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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Anti-CD1a antibody [EP3622] (ab108309) at 1/1000 dilution (Purified) + Human thymus lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 37 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?The molecular weight observed is consistent with what has been described in the literature (PMID: 18178838).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab108309 has been referenced in 5 publications.
- Hu YQ & Zhang JZ A Comparison for Type 2 Cytokines and Lesional Inflammatory Infiltrations in Bullous Pemphigoid and Atopic Dermatitis. Clin Cosmet Investig Dermatol 15:2313-2321 (2022). PubMed: 36325102
- Bolognesi MM et al. Multiplex Staining by Sequential Immunostaining and Antibody Removal on Routine Tissue Sections. J Histochem Cytochem 65:431-444 (2017). PubMed: 28692376
- Nguyen N et al. Tumor infiltrating lymphocytes and survival in patients with head and neck squamous cell carcinoma. Head Neck 38:1074-84 (2016). Human . PubMed: 26879675
- Tam J et al. Reconstitution of full-thickness skin by microcolumn grafting. J Tissue Eng Regen Med N/A:N/A (2016). IHC-P ; Human . PubMed: 27296503
- Perelygina L et al. Rubella persistence in epidermal keratinocytes and granuloma M2 macrophages in patients with primary immunodeficiencies. J Allergy Clin Immunol 138:1436-1439.e11 (2016). IF ; Human . PubMed: 27613149