Recombinant
RabMAb

Recombinant Anti-CD1a antibody [EP3622] - BSA and Azide free (ab217214)

Overview

  • Product name

    Anti-CD1a antibody [EP3622] - BSA and Azide free
    See all CD1a primary antibodies
  • Description

    Rabbit monoclonal [EP3622] to CD1a - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in human CD1a

  • Positive control

    • Human fetal thymus lysate, human T-78 cell lysate, human skin tissue.
  • General notes

    Ab217214 is the carrier-free version of ab108309. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab217214 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at 4°C (up to 6 months). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Dissociation constant (KD)

    KD = 1.30 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EP3622
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab217214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.
  • Tissue specificity

    Expressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell leukemias, and in various other tissues.
  • Sequence similarities

    Contains 1 Ig-like (immunoglobulin-like) domain.
  • Cellular localization

    Cell membrane. Endosome membrane. Subject to intracellular trafficking between the cell membrane and endosomes. Localizes to cell surface lipid rafts.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 1a antibody
    • CD1 antibody
    • CD1a antibody
    • CD1A Antigen antibody
    • CD1A antigen, a polypeptide antibody
    • CD1a molecule antibody
    • CD1A_HUMAN antibody
    • cluster of differentiation 1 A antibody
    • cortical thymocyte antigen CD1A antibody
    • differentiation antigen CD1 alpha 3 antibody
    • epidermal dendritic cell marker CD1a antibody
    • FCB 6 antibody
    • FCB6 antibody
    • HTA 1 antibody
    • HTA1 antibody
    • hTa1 thymocyte antigen antibody
    • OTTHUMP00000018907 antibody
    • R 4 antibody
    • R4 antibody
    • T 6 antibody
    • T-cell surface antigen T6/Leu-6 antibody
    • T-cell surface glycoprotein CD1a antibody
    • T6 antibody
    see all

Images

  • ab108309, at 1/250, staining CD1a in human skin tissue by immunohistochemistry

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108309).

  • ab108309 showing negative staining in Normal stomach tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108309).

  • ab108309 showing negative staining in Normal uterus tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108309).

  • ab108309 showing positive staining in Normal tonsil tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108309).

  • ab108309 showing negative staining in Normal breast tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108309).

  • ab108309 showing negative staining in Normal kidney tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108309).

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108309).

References

This product has been referenced in:

  • Nguyen N  et al. Tumor infiltrating lymphocytes and survival in patients with head and neck squamous cell carcinoma. Head Neck 38:1074-84 (2016). Human . Read more (PubMed: 26879675) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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