Anti-CD1a antibody [NA1/34] - BSA and Azide free (ab244579)
Key features and details
- Mouse monoclonal [NA1/34] to CD1a - BSA and Azide free
- Suitable for: IHC-Fr, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-CD1a antibody [NA1/34] - BSA and Azide free
See all CD1a primary antibodies -
Description
Mouse monoclonal [NA1/34] to CD1a - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: IHC-Fr, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD1a. Human thymocytes
-
Positive control
- IHC-Fr: Normal human skin. ICC: MOLT-4 and Jurkat cells.
-
General notes
ab244579 is a PBS-only buffer format of ab238463. Please refer to ab238463 for recommended dilutions, protocols, and image data.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
NA1/34 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Alternative Versions
-
Conjugation kits
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab244579 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-Fr | Use at an assay dependent concentration. | |
ICC/IF | Use a concentration of 1 - 5 µg/ml. |
Target
-
Function
Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. -
Tissue specificity
Expressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell leukemias, and in various other tissues. -
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. Endosome membrane. Subject to intracellular trafficking between the cell membrane and endosomes. Localizes to cell surface lipid rafts. - Information by UniProt
-
Database links
- Entrez Gene: 909 Human
- Omim: 188370 Human
- SwissProt: P06126 Human
- Unigene: 1309 Human
-
Alternative names
- CD 1a antibody
- CD1 antibody
- CD1a antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-CD1a antibody [NA1/34] - BSA and Azide free (ab244579)
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab238463)
ab238463 staining CD1a in MOLT-4 cells. The cells were fixed with 4% PFA (10min), permeabilized with 0.1%PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab238463 at 1μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
-
Immunohistochemistry (Frozen sections) - Anti-CD1a antibody [NA1/34] - BSA and Azide free (ab244579)
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab238463).
IHC image of CD1a staining in a section of frozen normal human skin*.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab238463 (shown in green) at 1 μg/ml. The section was then incubated with ab150117 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 488, 1/1000)) presabsorbed for 1 hour at room temperature. The secondary-only control image is taken from an identical assay without primary antibody. The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
-
Immunocytochemistry/ Immunofluorescence - Anti-CD1a antibody [NA1/34] - BSA and Azide free (ab244579)
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab238463)
ab238463 staining CD1a in Jurkat cells. The cells were fixed with 4% PFA (10min), permeabilized with 0.1%PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab238463 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
-
Immunocytochemistry/ Immunofluorescence - Anti-CD1a antibody [NA1/34] - BSA and Azide free (ab244579)
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab238463)
ab238463 showing negative staining for CD1a in U2OS cells (NEGATIVE CELL LINE). The cells were fixed with 4% PFA (10min), permeabilized with 0.1%PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab238463 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (1)
ab244579 has been referenced in 1 publication.
- Bradstock KF et al. Subpopulations of normal and leukemic human thymocytes: an analysis with the use of monoclonal antibodies. J Natl Cancer Inst 65:33-42 (1980). PubMed: 6993744