Recombinant
RabMAb

Recombinant Anti-CD1c antibody [EPR23189-305] (ab273049)

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Overview

  • Product name

    Anti-CD1c antibody [EPR23189-305]
    See all CD1c primary antibodies

  • Description

    Rabbit monoclonal [EPR23189-305] to CD1c

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P,IP or WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human CD1c aa 1-300. The exact sequence is proprietary.
    Database link: P29017

  • Positive control

    • ICC/IF: Jurkat cells. FC: Jurkat cells; Human PBMCs.

  • General notes

    Note: Clone EPR23189-305 (this product) is a different clone to that of ab246520.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23189-305

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab273049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.
ICC/IF 1/100.
  • Application notes
    Is unsuitable for IHC-P,IP or WB.

    • Target

    • Function

      Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.

    • Tissue specificity

      Expressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues.

    • Sequence similarities

      Contains 1 Ig-like (immunoglobulin-like) domain.

    • Cellular localization

      Cell membrane. Endosome membrane. Subject to intracellular trafficking between the cell membrane and endosomes.
    • Information by UniProt

    • Database links

      • Alternative names

        • BDCA1 antibody
        • CD1 antibody
        • CD1A antibody
        • CD1c antibody
        • CD1c antigen antibody
        • CD1C antigen c polypeptide antibody
        • CD1c molecule antibody
        • CD1C_HUMAN antibody
        • Cortical thymocyte antigen CD1C antibody
        • Differentiation antigen CD1 alpha 3 antibody
        • R7 antibody
        • T cell surface glycoprotein CD1c antibody
        • T-cell surface glycoprotein CD1c antibody
        see all

      Images

      • Flow Cytometry - Anti-CD1c antibody [EPR23189-305] (ab273049)
        Flow Cytometry - Anti-CD1c antibody [EPR23189-305] (ab273049)

        Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD1c with ab273049 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Cells were stained with rabbit IgG (Left) or ab273049 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor® 647.

        Gated on viable cells.

      • Immunocytochemistry/ Immunofluorescence - Anti-CD1c antibody [EPR23189-305] (ab273049)
        Immunocytochemistry/ Immunofluorescence - Anti-CD1c antibody [EPR23189-305] (ab273049)

        Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Jurkat cells labelling CD1c with ab273049 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in Jurkat cell line. Negative control: THP-1 (PMID: 24935257). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).

        Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

      • Flow Cytometry - Anti-CD1c antibody [EPR23189-305] (ab273049)
        Flow Cytometry - Anti-CD1c antibody [EPR23189-305] (ab273049)

        Flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte)(Left) / Jurkat (Human T cell leukemia T lymphocyte)(Right) cells labelling CD1c with ab273049 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

        Negative control: THP-1 (PMID: 24935257).

        Gated on viable cells.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References

      Publishing research using ab273049? Please let us know so that we can cite the reference in this datasheet.

      ab273049 has not yet been referenced specifically in any publications.

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