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    cd1c-antibody-epr23189-305-ab273049.pdf

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Immunology Adaptive Immunity T Cells CD
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-CD1c antibody [EPR23189-305] (ab273049)

  • Datasheet
  • Certificate of Compliance
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Flow Cytometry - Anti-CD1c antibody [EPR23189-305] (ab273049)
  • Flow Cytometry - Anti-CD1c antibody [EPR23189-305] (ab273049)
  • Flow Cytometry - Anti-CD1c antibody [EPR23189-305] (ab273049)
  • Anti-CD1c antibody [EPR23189-305] (ab273049)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23189-305] to CD1c
  • Suitable for: Flow Cyt
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Human CD1C knockout Jurkat cell line (ab273867)
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Anti-KCNN4 antibody (ab75956)

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Overview

  • Product name

    Anti-CD1c antibody [EPR23189-305]
    See all CD1c primary antibodies
  • Description

    Rabbit monoclonal [EPR23189-305] to CD1c
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P,IP or WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: Jurkat cells; Human PBMCs.
  • General notes

    Note: Clone EPR23189-305 (this product) is a different clone to that of ab246520.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR23189-305
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Immunology
    • Immunoglobulins
    • Receptors

Associated products

  • Alternative Versions

    • Anti-CD1c antibody [EPR23189-305] - BSA and Azide free (ab273060)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human CD1C knockout Jurkat cell line (ab273867)
  • Related Products

    • Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab273049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
1/500.

We recommend to use this product for cell-surface staining only

Notes
Flow Cyt
1/500.

We recommend to use this product for cell-surface staining only

Application notes
Is unsuitable for ICC/IF,IHC-P,IP or WB.

Target

  • Function

    Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.
  • Tissue specificity

    Expressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues.
  • Sequence similarities

    Contains 1 Ig-like (immunoglobulin-like) domain.
  • Cellular localization

    Cell membrane. Endosome membrane. Subject to intracellular trafficking between the cell membrane and endosomes.
  • Target information above from: UniProt accession P29017 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 911 Human
    • Omim: 188340 Human
    • SwissProt: P29017 Human
    • Unigene: 132448 Human
    • Alternative names

      • BDCA1 antibody
      • CD1 antibody
      • CD1A antibody
      • CD1c antibody
      • CD1c antigen antibody
      • CD1C antigen c polypeptide antibody
      • CD1c molecule antibody
      • CD1C_HUMAN antibody
      • Cortical thymocyte antigen CD1C antibody
      • Differentiation antigen CD1 alpha 3 antibody
      • R7 antibody
      • T cell surface glycoprotein CD1c antibody
      • T-cell surface glycoprotein CD1c antibody
      see all

    Images

    • Flow Cytometry - Anti-CD1c antibody [EPR23189-305] (ab273049)
      Flow Cytometry - Anti-CD1c antibody [EPR23189-305] (ab273049)

      Flow cytometry overlay histogram histogram showing wild-type (green line) and CD1C knockout Jurkat cells stained with ab273049 (red line). The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab273049) (1x106 in 100 µl at 0.2 µg/ml) for 30 min at 4°C.

      The secondary antibody Goat anti-rabbit IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150081) was used at 1/2000 for 30 min at 4°C.

      Isotype control antibody was Rabbit IgG (monoclonal) (ab172730) used at the same concentration and conditions as the primary antibody (wild-type Jurkat - black line; CD1C knockout Jurkat - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

      Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

    • Flow Cytometry - Anti-CD1c antibody [EPR23189-305] (ab273049)
      Flow Cytometry - Anti-CD1c antibody [EPR23189-305] (ab273049)

      Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD1c with ab273049 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Cells were stained with rabbit IgG (Left) or ab273049 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor® 647.

      Gated on viable cells.

    • Flow Cytometry - Anti-CD1c antibody [EPR23189-305] (ab273049)
      Flow Cytometry - Anti-CD1c antibody [EPR23189-305] (ab273049)

      Flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte)(Left) / Jurkat (Human T cell leukemia T lymphocyte)(Right) cells labelling CD1c with ab273049 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

      Negative control: THP-1 (PMID: 24935257).

      Gated on viable cells.

    • Anti-CD1c antibody [EPR23189-305] (ab273049)
      Anti-CD1c antibody [EPR23189-305] (ab273049)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab273049? Please let us know so that we can cite the reference in this datasheet.

    ab273049 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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