Recombinant Anti-CD1c antibody [EPR23189-305] (ab273049)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23189-305] to CD1c
- Suitable for: Flow Cyt
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD1c antibody [EPR23189-305]
See all CD1c primary antibodies -
Description
Rabbit monoclonal [EPR23189-305] to CD1c -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P,IP or WB -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: Jurkat cells; Human PBMCs.
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General notes
Note: Clone EPR23189-305 (this product) is a different clone to that of ab246520.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23189-305 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab273049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
1/500.
We recommend to use this product for cell-surface staining only |
Notes |
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Flow Cyt
1/500. We recommend to use this product for cell-surface staining only |
Target
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Function
Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. -
Tissue specificity
Expressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues. -
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. Endosome membrane. Subject to intracellular trafficking between the cell membrane and endosomes. - Information by UniProt
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Database links
- Entrez Gene: 911 Human
- Omim: 188340 Human
- SwissProt: P29017 Human
- Unigene: 132448 Human
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Alternative names
- BDCA1 antibody
- CD1 antibody
- CD1A antibody
see all
Images
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Flow cytometry overlay histogram histogram showing wild-type (green line) and CD1C knockout Jurkat cells stained with ab273049 (red line). The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab273049) (1x106 in 100 µl at 0.2 µg/ml) for 30 min at 4°C.
The secondary antibody Goat anti-rabbit IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150081) was used at 1/2000 for 30 min at 4°C.
Isotype control antibody was Rabbit IgG (monoclonal) (ab172730) used at the same concentration and conditions as the primary antibody (wild-type Jurkat - black line; CD1C knockout Jurkat - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
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Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD1c with ab273049 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Cells were stained with rabbit IgG (Left) or ab273049 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor® 647.
Gated on viable cells.
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Flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte)(Left) / Jurkat (Human T cell leukemia T lymphocyte)(Right) cells labelling CD1c with ab273049 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Negative control: THP-1 (PMID: 24935257).
Gated on viable cells.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab273049 has not yet been referenced specifically in any publications.