Overview

  • Product name

    Anti-CD1c antibody [OTI2F4]
    See all CD1c primary antibodies
  • Description

    Mouse monoclonal [OTI2F4] to CD1c
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human, Cynomolgus monkey
  • Immunogen

    Full length human recombinant protein of human CD1C(NP_001756) produced in HEK293T cell.

  • General notes

    The clone number has been updated from 2F4 to OTI2F4, both clone numbers name the same clone.

    This product was changed from ascites to tissue culture supernatant on 29th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from TCS
  • Clonality

    Monoclonal
  • Clone number

    OTI2F4
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab156708 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 38 kDa.
IHC-P 1/150.

Target

  • Function

    Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.
  • Tissue specificity

    Expressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues.
  • Sequence similarities

    Contains 1 Ig-like (immunoglobulin-like) domain.
  • Cellular localization

    Cell membrane. Endosome membrane. Subject to intracellular trafficking between the cell membrane and endosomes.
  • Information by UniProt
  • Database links

  • Alternative names

    • BDCA1 antibody
    • CD1 antibody
    • CD1A antibody
    • CD1c antibody
    • CD1c antigen antibody
    • CD1C antigen c polypeptide antibody
    • CD1c molecule antibody
    • CD1C_HUMAN antibody
    • Cortical thymocyte antigen CD1C antibody
    • Differentiation antigen CD1 alpha 3 antibody
    • R7 antibody
    • T cell surface glycoprotein CD1c antibody
    • T-cell surface glycoprotein CD1c antibody
    see all

Images

  • Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-CD1C mouse monoclonal antibody(ab156708)
  • All lanes : Anti-CD1c antibody [OTI2F4] (ab156708)

    Lane 1 : HEK293T cells were transfected with the pCMV6-ENTRY
    Lane 2 : HEK293T cells were transfected with pCMV6-ENTRY CD1C

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 38 kDa



    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC211378)
  • ab156708 staining MAFA in Cynomolgus monkey lymph node sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% donkey serum for 20 minutes at romm temperature; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50) for 30 minutes at room temperature. A Biotin-conjugated Donkey anti-mouse IgG polyclonal (1/2000) was used as the secondary antibody.

    See Abreview

  • Immunohistochemical staining of paraffin-embedded Human tonsil using anti-CD1C mouse monoclonal antibody. (ab156708)

References

This product has been referenced in:

  • Scutera S  et al. Adaptive Regulation of Osteopontin Production by Dendritic Cells Through the Bidirectional Interaction With Mesenchymal Stromal Cells. Front Immunol 9:1207 (2018). Read more (PubMed: 29910810) »
  • Chen J  et al. Regulatory T cells with a defect in inhibition on co-stimulation deteriorated primary biliary cholangitis. Oncotarget 8:108406-108417 (2017). Read more (PubMed: 29312539) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lymph node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA
Permeabilization
No
Specification
Lymph node
Blocking step
Casein as blocking agent for 15 minute(s) · Concentration: 0.25% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Aug 03 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0 Bond Epitope Retrieval Solution 1 (ER1, Leica)
Permeabilization
No
Specification
Spleen
Blocking step
2.5% Normal Horse Serum - VectorLabs ImmPRESS™ Excel Amplified HRP Polymer Staining Kit (Anti-Mouse Ig) as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: RT°C
Fixative
10% Neutral Buffered Formalin

Abcam user community

Verified customer

Submitted Jun 15 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated
Sample
Cynomolgus Monkey Tissue sections (Lymph node)
Specification
Lymph node
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: Rt°C
Fixative
Formaldehyde

Jing Ma

Verified customer

Submitted Feb 07 2014

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