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    cd1c-antibody-oti2f4-ab156708.pdf

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Immunology Adaptive Immunity T Cells CD
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Anti-CD1c antibody [OTI2F4] (ab156708)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (5)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [OTI2F4] (ab156708)
  • Western blot - Anti-CD1c antibody [OTI2F4] (ab156708)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [OTI2F4] (ab156708)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [OTI2F4] (ab156708)

Key features and details

  • Mouse monoclonal [OTI2F4] to CD1c
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-CD1c antibody [OTI2F4]
    See all CD1c primary antibodies
  • Description

    Mouse monoclonal [OTI2F4] to CD1c
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Full length human recombinant protein of human CD1C(NP_001756) produced in HEK293T cell.

  • General notes

    The clone number has been updated from 2F4 to OTI2F4, both clone numbers name the same clone.

    This product was changed from ascites to tissue culture supernatant on 29th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from TCS
  • Clonality

    Monoclonal
  • Clone number

    OTI2F4
  • Isotype

    IgG1
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Immunology
    • Immunoglobulins
    • Receptors

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab156708 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
Application Abreviews Notes
WB
1/2000. Predicted molecular weight: 38 kDa.
IHC-P (3)
1/150.
Notes
WB
1/2000. Predicted molecular weight: 38 kDa.
IHC-P
1/150.

Target

  • Function

    Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.
  • Tissue specificity

    Expressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues.
  • Sequence similarities

    Contains 1 Ig-like (immunoglobulin-like) domain.
  • Cellular localization

    Cell membrane. Endosome membrane. Subject to intracellular trafficking between the cell membrane and endosomes.
  • Target information above from: UniProt accession P29017 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 911 Human
    • Omim: 188340 Human
    • SwissProt: P29017 Human
    • Unigene: 132448 Human
    • Alternative names

      • BDCA1 antibody
      • CD1 antibody
      • CD1A antibody
      • CD1c antibody
      • CD1c antigen antibody
      • CD1C antigen c polypeptide antibody
      • CD1c molecule antibody
      • CD1C_HUMAN antibody
      • Cortical thymocyte antigen CD1C antibody
      • Differentiation antigen CD1 alpha 3 antibody
      • R7 antibody
      • T cell surface glycoprotein CD1c antibody
      • T-cell surface glycoprotein CD1c antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [OTI2F4] (ab156708)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [OTI2F4] (ab156708)
      Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-CD1C mouse monoclonal antibody(ab156708)
    • Western blot - Anti-CD1c antibody [OTI2F4] (ab156708)
      Western blot - Anti-CD1c antibody [OTI2F4] (ab156708)
      All lanes : Anti-CD1c antibody [OTI2F4] (ab156708)

      Lane 1 : HEK293T cells were transfected with the pCMV6-ENTRY
      Lane 2 : HEK293T cells were transfected with pCMV6-ENTRY CD1C

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 38 kDa



      HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC211378)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [OTI2F4] (ab156708)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [OTI2F4] (ab156708)This image is courtesy of an Abreview submitted by Jing Ma

      ab156708 staining MAFA in Cynomolgus monkey lymph node sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% donkey serum for 20 minutes at romm temperature; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50) for 30 minutes at room temperature. A Biotin-conjugated Donkey anti-mouse IgG polyclonal (1/2000) was used as the secondary antibody.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [OTI2F4] (ab156708)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [OTI2F4] (ab156708)
      Immunohistochemical staining of paraffin-embedded Human tonsil using anti-CD1C mouse monoclonal antibody. (ab156708)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (5)

    Publishing research using ab156708? Please let us know so that we can cite the reference in this datasheet.

    ab156708 has been referenced in 5 publications.

    • Somri-Gannam L  et al. IGF1R Axis Inhibition Restores Dendritic Cell Antitumor Response in Ovarian Cancer. Transl Oncol 13:100790 (2020). PubMed: 32428851
    • Mattiola I  et al. The macrophage tetraspan MS4A4A enhances dectin-1-dependent NK cell-mediated resistance to metastasis. Nat Immunol 20:1012-1022 (2019). PubMed: 31263276
    • Rashid R  et al. Highly multiplexed immunofluorescence images and single-cell data of immune markers in tonsil and lung cancer. Sci Data 6:323 (2019). PubMed: 31848351
    • Scutera S  et al. Adaptive Regulation of Osteopontin Production by Dendritic Cells Through the Bidirectional Interaction With Mesenchymal Stromal Cells. Front Immunol 9:1207 (2018). PubMed: 29910810
    • Chen J  et al. Regulatory T cells with a defect in inhibition on co-stimulation deteriorated primary biliary cholangitis. Oncotarget 8:108406-108417 (2017). PubMed: 29312539

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD1c antibody [2F4]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Lymph node)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA
    Permeabilization
    No
    Specification
    Lymph node
    Blocking step
    Casein as blocking agent for 15 minute(s) · Concentration: 0.25% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 03 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD1c antibody [2F4]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rhesus monkey Tissue sections (Spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0 Bond Epitope Retrieval Solution 1 (ER1, Leica)
    Permeabilization
    No
    Specification
    Spleen
    Blocking step
    2.5% Normal Horse Serum - VectorLabs ImmPRESS™ Excel Amplified HRP Polymer Staining Kit (Anti-Mouse Ig) as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: RT°C
    Fixative
    10% Neutral Buffered Formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 15 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD1c [2F4] antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Antigen retrieval step
    Heat mediated
    Sample
    Cynomolgus Monkey Tissue sections (Lymph node)
    Specification
    Lymph node
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: Rt°C
    Fixative
    Formaldehyde
    Read More

    Jing Ma

    Verified customer

    Submitted Feb 07 2014

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