Anti-CD1c antibody [OTI2F4] (ab156708)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [OTI2F4] (ab156708)](https://www.abcam.com/ps/products/156/ab156708/Images/ab156708-163813-anti-cd1c-antibody-oti2f4-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [OTI2F4] (ab156708)")
Key features and details
- Mouse monoclonal [OTI2F4] to CD1c
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD1c antibody [OTI2F4]
See all CD1c primary antibodies -
Description
Mouse monoclonal [OTI2F4] to CD1c -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to CD1c. produced in HEK293T cell
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General notes
The clone number has been updated from 2F4 to OTI2F4, both clone numbers name the same clone.
This product was changed from ascites to tissue culture supernatant on 29th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
OTI2F4 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156708 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000. Predicted molecular weight: 38 kDa.
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| IHC-P | (4) |
1/150.
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| Notes |
|---|
|
WB
1/2000. Predicted molecular weight: 38 kDa. |
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IHC-P
1/150. |
Target
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Function
Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. -
Tissue specificity
Expressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues. -
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. Endosome membrane. Subject to intracellular trafficking between the cell membrane and endosomes. - Information by UniProt
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Database links
- Entrez Gene: 911 Human
- Omim: 188340 Human
- SwissProt: P29017 Human
- Unigene: 132448 Human
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Alternative names
- BDCA1 antibody
- CD1 antibody
- CD1A antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [OTI2F4] (ab156708)Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-CD1C mouse monoclonal antibody(ab156708)
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![Western blot - Anti-CD1c antibody [OTI2F4] (ab156708)](https://www.abcam.com/ps/products/156/ab156708/Images/ab156708-163812-anti-cd1c-antibody-oti2f4-western-blot.jpg)
Western blot - Anti-CD1c antibody [OTI2F4] (ab156708)All lanes : Anti-CD1c antibody [OTI2F4] (ab156708)
Lane 1 : HEK293T cells were transfected with the pCMV6-ENTRY
Lane 2 : HEK293T cells were transfected with pCMV6-ENTRY CD1C
Lysates/proteins at 5 µg per lane.
Predicted band size: 38 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC211378) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [OTI2F4] (ab156708)](https://www.abcam.com/ps/products/156/ab156708/Images/ab156708-277445-anti-cd1c-antibody-oti2f4-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [OTI2F4] (ab156708)This image is courtesy of an Abreview submitted by Jing Maab156708 staining MAFA in Cynomolgus monkey lymph node sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% donkey serum for 20 minutes at romm temperature; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50) for 30 minutes at room temperature. A Biotin-conjugated Donkey anti-mouse IgG polyclonal (1/2000) was used as the secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [OTI2F4] (ab156708)](https://www.abcam.com/ps/products/156/ab156708/Images/ab156708-163811-anti-cd1c-antibody-oti2f4-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [OTI2F4] (ab156708)Immunohistochemical staining of paraffin-embedded Human tonsil using anti-CD1C mouse monoclonal antibody. (ab156708)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab156708 has been referenced in 7 publications.
- Somri-Gannam L et al. IGF1R Axis Inhibition Restores Dendritic Cell Antitumor Response in Ovarian Cancer. Transl Oncol 13:100790 (2020). PubMed: 32428851
- van Beek JJP et al. Human pDCs Are Superior to cDC2s in Attracting Cytolytic Lymphocytes in Melanoma Patients Receiving DC Vaccination. Cell Rep 30:1027-1038.e4 (2020). PubMed: 31995747
- Molgora M et al. TREM2 Modulation Remodels the Tumor Myeloid Landscape Enhancing Anti-PD-1 Immunotherapy. Cell 182:886-900.e17 (2020). PubMed: 32783918
- Mattiola I et al. The macrophage tetraspan MS4A4A enhances dectin-1-dependent NK cell-mediated resistance to metastasis. Nat Immunol 20:1012-1022 (2019). PubMed: 31263276
- Rashid R et al. Highly multiplexed immunofluorescence images and single-cell data of immune markers in tonsil and lung cancer. Sci Data 6:323 (2019). PubMed: 31848351
- Scutera S et al. Adaptive Regulation of Osteopontin Production by Dendritic Cells Through the Bidirectional Interaction With Mesenchymal Stromal Cells. Front Immunol 9:1207 (2018). PubMed: 29910810
- Chen J et al. Regulatory T cells with a defect in inhibition on co-stimulation deteriorated primary biliary cholangitis. Oncotarget 8:108406-108417 (2017). PubMed: 29312539