Product nameAnti-CD1d antibody [EPR22526-72]
See all CD1d primary antibodies
DescriptionRabbit monoclonal [EPR22526-72] to CD1d
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Human
Recombinant fragment within Human CD1d aa 1-300. The exact sequence is proprietary.
Database link: P15813
- WB: Human lymph node and thymus lysates; Jurkat and MOLT-4 whole cell lysates. IP: Jurkat whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab229903 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 38 kDa).|
FunctionAntigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells.
Tissue specificityExpressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues.
Sequence similaritiesContains 1 Ig-like (immunoglobulin-like) domain.
Cellular localizationCell membrane. Endosome membrane. Lysosome membrane. Subject to intracellular trafficking between the cell membrane, endosomes and lysosomes.
- Information by UniProt
- Antigen-presenting glycoprotein CD1d antibody
- CD1.1 antibody
- CD1A antibody
All lanes : Anti-CD1d antibody [EPR22526-72] (ab229903) at 1/1000 dilution
Lane 1 : Human lymph node lysate
Lane 2 : Human thymus lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
Lane 5 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 6 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 38 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Exposure time: Lane 1: 3 minutes; Lanes 2-6: 48 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 10809957).
Negative control: SH-SY5Y (PMID: 26749374), K562 (PMID: 21037579).
CD1d was immunoprecipitated from 0.35 mg of Jurkat (human T cell leukemia cell line from peripheral blood) whole lysate with ab229903 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229903 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.
Lane 1: Jurkat whole cell lysate 10 µg (Input).
Lane 2: ab229903 IP in Jurkat whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229903 in Jurkat whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
ab229903 has not yet been referenced specifically in any publications.