Recombinant
RabMAb

Recombinant Anti-CD1d antibody [EPR22526-72] (ab229903)

Overview

  • Product name

    Anti-CD1d antibody [EPR22526-72]
    See all CD1d primary antibodies
  • Description

    Rabbit monoclonal [EPR22526-72] to CD1d
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human CD1d aa 1-300. The exact sequence is proprietary.
    Database link: P15813

  • Positive control

    • WB: Human lymph node and thymus lysates; Jurkat and MOLT-4 whole cell lysates. IP: Jurkat whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab229903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 38 kDa).
IP 1/30.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells.
    • Tissue specificity

      Expressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues.
    • Sequence similarities

      Contains 1 Ig-like (immunoglobulin-like) domain.
    • Cellular localization

      Cell membrane. Endosome membrane. Lysosome membrane. Subject to intracellular trafficking between the cell membrane, endosomes and lysosomes.
    • Information by UniProt
    • Database links

    • Alternative names

      • Antigen-presenting glycoprotein CD1d antibody
      • CD1.1 antibody
      • CD1A antibody
      • CD1d antibody
      • CD1D antigen antibody
      • CD1D antigen d polypeptide antibody
      • CD1d molecule antibody
      • CD1D_HUMAN antibody
      • Cd1d1 antibody
      • differentiation antigen CD1 alpha 3 antibody
      • HMC class I antigen like glycoprotein CD1D antibody
      • Ly 38 antibody
      • MGC34622 antibody
      • R3 antibody
      • R3G1 antibody
      • T cell surface glycoprotein CD1d antibody
      • Thymocyte antigen CD1D antibody
      see all

    Images

    • All lanes : Anti-CD1d antibody [EPR22526-72] (ab229903) at 1/1000 dilution

      Lane 1 : Human lymph node lysate
      Lane 2 : Human thymus lysate
      Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
      Lane 4 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
      Lane 5 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
      Lane 6 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 38 kDa
      Observed band size: 48 kDa
      why is the actual band size different from the predicted?



      Exposure time: Lane 1: 3 minutes; Lanes 2-6: 48 seconds.

      Blocking/Dilution buffer: 5% NFDM/TBST.

      The molecular weight observed is consistent with what has been described in the literature (PMID: 10809957).

      Negative control: SH-SY5Y (PMID: 26749374), K562 (PMID: 21037579).

    • CD1d was immunoprecipitated from 0.35 mg of Jurkat (human T cell leukemia cell line from peripheral blood) whole lysate with ab229903 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229903 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

      Lane 1: Jurkat whole cell lysate 10 µg (Input). 

      Lane 2: ab229903 IP in Jurkat whole cell lysate. 

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229903 in Jurkat whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 30 seconds.

    References

    ab229903 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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