Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22526-72] to CD1d
- Suitable for: WB, IP
- Reacts with: Human
Product nameAnti-CD1d antibody [EPR22526-72]
See all CD1d primary antibodies
DescriptionRabbit monoclonal [EPR22526-72] to CD1d
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Human
Recombinant fragment within Human CD1d aa 1-300. The exact sequence is proprietary.
Database link: P15813
- WB: Human lymph node and thymus lysates; Jurkat and MOLT-4 whole cell lysates. IP: Jurkat whole cell lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab229903 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 38 kDa).|
FunctionAntigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells.
Tissue specificityExpressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues.
Sequence similaritiesContains 1 Ig-like (immunoglobulin-like) domain.
Cellular localizationCell membrane. Endosome membrane. Lysosome membrane. Subject to intracellular trafficking between the cell membrane, endosomes and lysosomes.
- Information by UniProt
- Antigen-presenting glycoprotein CD1d antibody
- CD1.1 antibody
- CD1A antibody
All lanes : Anti-CD1d antibody [EPR22526-72] (ab229903) at 1/1000 dilution
Lane 1 : Human lymph node lysate
Lane 2 : Human thymus lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
Lane 5 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 6 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 38 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Exposure time: Lane 1: 3 minutes; Lanes 2-6: 48 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 10809957).
Negative control: SH-SY5Y (PMID: 26749374), K562 (PMID: 21037579).
CD1d was immunoprecipitated from 0.35 mg of Jurkat (human T cell leukemia cell line from peripheral blood) whole lysate with ab229903 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229903 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.
Lane 1: Jurkat whole cell lysate 10 µg (Input).
Lane 2: ab229903 IP in Jurkat whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229903 in Jurkat whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229903 has not yet been referenced specifically in any publications.