Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22526-9] to CD1d
- Suitable for: IP, WB, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
Product nameAnti-CD1d antibody [EPR22526-9]
See all CD1d primary antibodies
DescriptionRabbit monoclonal [EPR22526-9] to CD1d
Tested applicationsSuitable for: IP, WB, Flow Cytmore details
Unsuitable for: ICC/IF or IHC-P
Species reactivityReacts with: Human
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
- WB: Jurkat and MOLT-4 whole cell lysate. IP: Jurkat whole cell lysate. Flow Cyt: Human peripheral blood mononuclear and Jurkat cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein A purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab256344 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 38 kDa.
1/1000. Predicted molecular weight: 38 kDa.
FunctionAntigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells.
Tissue specificityExpressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues.
Sequence similaritiesContains 1 Ig-like (immunoglobulin-like) domain.
Cellular localizationCell membrane. Endosome membrane. Lysosome membrane. Subject to intracellular trafficking between the cell membrane, endosomes and lysosomes.
- Information by UniProt
- Antigen-presenting glycoprotein CD1d antibody
- CD1.1 antibody
- CD1A antibody
All lanes : Anti-CD1d antibody [EPR22526-9] (ab256344) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 2 : MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate
Lane 3 : SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate
Lane 4 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 38 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Exposure time: 37 seconds
The molecular weight observed is consistent with what has been described in the literature (PMID: 10809957).
Negative control: SH-SY5Y (PMID: 26749374), K562 (PMID: 21037579).
Blocking/Dilution buffer: 5% NFDM/TBST.
Flow cytometric analysis of K562 (human chronic myelogenous leukemia lymphoblast, Left) / Jurkat (human T cell leukemia T lymphocyte, Right) cells labeling CD1d with ab256344 at 1/600 dilution (0.1μg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
Negative control: K562 (PMID: 21037579).
Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling CD1d with ab256344 at 1/600 dilution (0.1μg) (Right) compared with a Rabbit monoclonal IgG (ab172730) (Left) isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG (Left) or ab256344 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor® 647. Gated on viable cells.
CD1d was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia T lymphocyte) whole cell lysate with ab256344 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256344 at 1/1000 dilution (0.6 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.
Lane 1: Jurkat whole cell lysate (10μg)
Lane 2: ab256344 IP in Jurkat whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256344 in Jurkat whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 mins.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab256344 has not yet been referenced specifically in any publications.