Overview

  • Product name

    Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)]
    See all CD1d primary antibodies
  • Description

    Mouse monoclonal [NOR3.2 (NOR3.2/13.17)] to CD1d
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, IHC-Fr, IHC-P, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Sheep, Human
  • Immunogen

    Fusion protein corresponding to Human CD1d.

  • Positive control

    • IHC-P: Human skin tissue. Flow Cyt: MOLT4 cells.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab11076 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells.
  • Tissue specificity

    Expressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues.
  • Sequence similarities

    Contains 1 Ig-like (immunoglobulin-like) domain.
  • Cellular localization

    Cell membrane. Endosome membrane. Lysosome membrane. Subject to intracellular trafficking between the cell membrane, endosomes and lysosomes.
  • Information by UniProt
  • Database links

  • Alternative names

    • Antigen-presenting glycoprotein CD1d antibody
    • CD1.1 antibody
    • CD1A antibody
    • CD1d antibody
    • CD1D antigen antibody
    • CD1D antigen d polypeptide antibody
    • CD1d molecule antibody
    • CD1D_HUMAN antibody
    • Cd1d1 antibody
    • differentiation antigen CD1 alpha 3 antibody
    • HMC class I antigen like glycoprotein CD1D antibody
    • Ly 38 antibody
    • MGC34622 antibody
    • R3 antibody
    • R3G1 antibody
    • T cell surface glycoprotein CD1d antibody
    • Thymocyte antigen CD1D antibody
    see all

Images

  • IHC image of CD1d staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA based pH 9.0 solution (epitope retrieval solution 2) for 20 mins. The section was then incubated with ab11076, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Overlay histogram showing MOLT4 cells stained with ab11076 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11076, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabeled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • ab11076 staining CD1d in human skin tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed with formaldehyde and an antigen retrieval step was performed using EDTA pH 8.0 for 20 minutes at 100°C. Samples were then incubated with ab11076 at a 1/200 dilution for 20 minutes at 25°C. The secondary used was an undiluted HRP conjugated goat anti-mouse/rabbit IgG.

    See Abreview

References

This product has been referenced in:

  • Ascierto ML  et al. Molecular signatures mostly associated with NK cells are predictive of relapse free survival in breast cancer patients. J Transl Med 11:145 (2013). IHC-P . Read more (PubMed: 23758773) »
  • Xu C  et al. Expression of CD1d and presence of invariant NKT cells in classical Hodgkin lymphoma. Am J Hematol 85:539-41 (2010). IHC . Read more (PubMed: 20575029) »
See all 7 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Specification
skin
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH 8, 20 min, 100C
Permeabilization
No

Abcam user community

Verified customer

Submitted Dec 12 2011

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (kidney)
Specification
kidney
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate acid
Permeabilization
Yes - triton x-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C

Mrs. Mari Palviainen

Verified customer

Submitted Mar 04 2011

Answer

Thank you for your enquiry regarding ab11076. For use in IHC (frozen) we recommend using this antibody at a 1:100 dilution or an approximate working concentration of 10ug/ml. However, we always suggest that the user titrates the antibody for use in their own system using the appropriate positive and negative controls to see what works best for them.

Read More

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