Recombinant
RabMAb

Recombinant Anti-CD1E antibody [EPR15746(B)] (ab187157)

Overview

  • Product name

    Anti-CD1E antibody [EPR15746(B)]
    See all CD1E primary antibodies
  • Description

    Rabbit monoclonal [EPR15746(B)] to CD1E
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CD1E aa 1-100. The exact sequence is proprietary.
    Database link: P15812

  • Positive control

    • HUVEC, HepG2 and Jurkat cell lysate. Raw264.7, NIH3T3 and PC-12 cell lysate. Human hepatocellular carcinoma tissue and rat kidney tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15746(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab187157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 44 kDa.
IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    T-cell surface glycoprotein CD1e, soluble binds diacetylated lipids, including phosphatidyl inositides and diacylated sulfoglycolipids, and is required for the presentation of glycolipid antigens on the cell surface. The membrane-associated form is not active.
  • Tissue specificity

    Expressed on cortical thymocytes, dendritic cells, Langerhans cells, on certain T-cell leukemias, and in various other tissues.
  • Sequence similarities

    Contains 1 Ig-like (immunoglobulin-like) domain.
  • Post-translational
    modifications

    Mono-ubiquitinated.
    Proteolytically cleaved in late endosomes to yield a soluble form.
  • Cellular localization

    Lysosome lumen and Golgi apparatus membrane. Early endosome. Late endosome. Predominantly localized in the trans-Golgi network in immature dendritic cells, and as a cleaved, soluble protein in the lysosome lumen of mature dendritic cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD_antigen=CD1e antibody
    • CD1A antibody
    • CD1e antibody
    • CD1e antigen antibody
    • CD1E antigen, e polypeptide antibody
    • CD1e molecule antibody
    • CD1E_HUMAN antibody
    • Differentiation antigen CD1-alpha-3 antibody
    • hCD1e antibody
    • Leukocyte differentiation antigen antibody
    • R2 antibody
    • R2G1 antibody
    • sCD1e antibody
    • soluble antibody
    • T-cell surface glycoprotein CD1e antibody
    • T-cell surface glycoprotein CD1e, membrane-associated antibody
    • T-cell surface glycoprotein CD1e, soluble antibody
    see all

Images

  • All lanes : Anti-CD1E antibody [EPR15746(B)] (ab187157) at 1/1000 dilution

    Lane 1 : HUVEC cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 44 kDa



    Produced under U.S. Patent No. 5,675,063.

  • All lanes : Anti-CD1E antibody [EPR15746(B)] (ab187157) at 1/1000 dilution

    Lane 1 : Raw264.7 cell lysate
    Lane 2 : PC-12 cell lysate
    Lane 3 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 44 kDa



    Produced under U.S. Patent No. 5,675,063.

  • Immunohistochemical analysis of paraffin embedded Human hepatocellular carcinoma tissue sections labeling CD1E using ab187157 at a 1/50 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

  • Immunohistochemical analysis of paraffin embedded rat kidney tissue sections labeling CD1E using ab187157 at a 1/50 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

References

ab187157 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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