Recombinant
RabMAb

Recombinant Anti-CD2 antibody [EPR6451] (ab131276)

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Overview

  • Product name

    Anti-CD2 antibody [EPR6451]
    See all CD2 primary antibodies

  • Description

    Rabbit monoclonal [EPR6451] to CD2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human CD2 aa 200-350. The exact sequence is proprietary.
    Database link: P06729

  • Positive control

    • Human thymus and Jurkat cell lysates. Human tonsil tissue and Human tonsil tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab131276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
Flow Cyt 1/20.

Target

  • Function

    CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD 2 antibody
      • CD2 antibody
      • CD2 antigen (p50), sheep red blood cell receptor antibody
      • CD2 antigen antibody
      • CD2 molecule antibody
      • CD2_HUMAN antibody
      • Erythrocyte receptor antibody
      • FLJ46032 antibody
      • LFA-2 antibody
      • LFA-3 receptor antibody
      • LFA2 antibody
      • LFA3 receptor antibody
      • Ly-37 antibody
      • Lymphocyte function antigen 2 antibody
      • lymphocyte-function antigen-2 antibody
      • OTTHUMP00000024366 antibody
      • Rosette receptor antibody
      • Sheep erythrocyte receptor antibody
      • SRBC antibody
      • T cell surface antigen CD2 antibody
      • T-cell surface antigen CD2 antibody
      • T-cell surface antigen T11/Leu-5 antibody
      • T-lymphocyte surface CD2 antigen antibody
      • T11 antibody
      see all

    Images

    • Flow Cytometry - Anti-CD2 antibody [EPR6451] (ab131276)
      Flow Cytometry - Anti-CD2 antibody [EPR6451] (ab131276)

      Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling CD2 with unpurified ab131276 at 1/20 dilution (red). The secondary antibody was Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

    • Western blot - Anti-CD2 antibody [EPR6451] (ab131276)
      Western blot - Anti-CD2 antibody [EPR6451] (ab131276)
      All lanes : Anti-CD2 antibody [EPR6451] (ab131276) at 1/1000 dilution

      Lane 1 : Human thymus lysate
      Lane 2 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 39 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)

      Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelled with ab131276 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)

      Immunohistochemical analysis of paraffin embedded Human colon tissue labelled with ab131276 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)

      ab131276 showing positive staining in Diffuse large B-cell lymphoma tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)

      ab131276 showing positive staining in Normal spleen tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)

      ab131276 showing positive staining in Hodgkin's lymphoma tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Santucci-Pereira J  et al. Genomic signature of parity in the breast of premenopausal women. Breast Cancer Res 21:46 (2019). Read more (PubMed: 30922380) »
    • Companioni Nápoles O  et al. SCHLAFEN 5 expression correlates with intestinal metaplasia that progresses to gastric cancer. J Gastroenterol 52:39-49 (2017). Read more (PubMed: 27032393) »
    See all 2 Publications for this product

    Publishing research using ab131276? Please let us know so that we can cite the reference in this datasheet.

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