Recombinant Anti-CD2 antibody [EPR6451] (ab131276)
![Flow Cytometry - Anti-CD2 antibody [EPR6451] (ab131276)](https://www.abcam.com/ps/products/131/ab131276/Images/ab131276-273058-anti-cd2-antibody-epr6451-flow-cytometry.jpg "Flow Cytometry - Anti-CD2 antibody [EPR6451] (ab131276)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6451] to CD2
- Suitable for: WB, IP, IHC-P, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-CD2 antibody [EPR6451]
See all CD2 primary antibodies -
Description
Rabbit monoclonal [EPR6451] to CD2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CD2 aa 200-350. The exact sequence is proprietary.
Database link: P06729 -
Positive control
- Human thymus and Jurkat cell lysates. Human tonsil tissue and Human tonsil tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6451 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
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| IP |
1/10 - 1/100.
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Flow Cyt |
1/20.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 39 kDa. |
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IP
1/10 - 1/100. |
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IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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Flow Cyt
1/20. |
Target
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Function
CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 914 Human
- Omim: 186990 Human
- SwissProt: P06729 Human
- Unigene: 523500 Human
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Alternative names
- CD 2 antibody
- CD2 antibody
- CD2 antigen (p50), sheep red blood cell receptor antibody
see all
Images
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Flow Cytometry - Anti-CD2 antibody [EPR6451] (ab131276)
Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling CD2 with unpurified ab131276 at 1/20 dilution (red). The secondary antibody was Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
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![Western blot - Anti-CD2 antibody [EPR6451] (ab131276)](https://www.abcam.com/ps/products/131/ab131276/Images/ab131276-145658-anti-cd2-antibody-epr6451-western-blot.jpg)
Western blot - Anti-CD2 antibody [EPR6451] (ab131276)All lanes : Anti-CD2 antibody [EPR6451] (ab131276) at 1/1000 dilution
Lane 1 : Human thymus lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 39 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)](https://www.abcam.com/ps/products/131/ab131276/Images/ab131276-145659-anti-cd2-antibody-epr6451-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelled with ab131276 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)](https://www.abcam.com/ps/products/131/ab131276/Images/ab131276-145657-anti-cd2-antibody-epr6451-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)Immunohistochemical analysis of paraffin embedded Human colon tissue labelled with ab131276 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)](https://www.abcam.com/ps/products/131/ab131276/Images/ab131276-145656-anti-cd2-antibody-epr6451-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)ab131276 showing positive staining in Diffuse large B-cell lymphoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)](https://www.abcam.com/ps/products/131/ab131276/Images/ab131276-145655-anti-cd2-antibody-epr6451-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)ab131276 showing positive staining in Normal spleen tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)](https://www.abcam.com/ps/products/131/ab131276/Images/ab131276-145654-anti-cd2-antibody-epr6451-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)ab131276 showing positive staining in Hodgkin's lymphoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-CD2 antibody [EPR6451] (ab131276)](https://www.abcam.com/ps/products/131/ab131276/Images/ab131276-8-benefits-of-recombinant-antibodies.png)
Anti-CD2 antibody [EPR6451] (ab131276)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab131276 has been referenced in 2 publications.
- Santucci-Pereira J et al. Genomic signature of parity in the breast of premenopausal women. Breast Cancer Res 21:46 (2019). PubMed: 30922380
- Companioni Nápoles O et al. SCHLAFEN 5 expression correlates with intestinal metaplasia that progresses to gastric cancer. J Gastroenterol 52:39-49 (2017). PubMed: 27032393