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Immunology Adaptive Immunity T Cells CD
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RecombinantRabMAb

Recombinant Anti-CD2 antibody [EPR6451] (ab131276)

  • Datasheet
  • SDS
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Flow Cytometry - Anti-CD2 antibody [EPR6451] (ab131276)
  • Western blot - Anti-CD2 antibody [EPR6451] (ab131276)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)
  • Anti-CD2 antibody [EPR6451] (ab131276)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6451] to CD2
  • Suitable for: WB, IP, IHC-P, Flow Cyt
  • Reacts with: Human

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Overview

  • Product name

    Anti-CD2 antibody [EPR6451]
    See all CD2 primary antibodies
  • Description

    Rabbit monoclonal [EPR6451] to CD2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD2 aa 200-350. The exact sequence is proprietary.
    Database link: P06729

  • Positive control

    • Human thymus and Jurkat cell lysates. Human tonsil tissue and Human tonsil tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR6451
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Human Lineage Negative
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • NK Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage

Associated products

  • Alternative Versions

    • Anti-CD2 antibody [EPR6451] - BSA and Azide free (ab248400)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Jurkat membrane extract lysate (ab30130)
  • Recombinant Protein

    • Recombinant Human CD2 protein (ab134855)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab131276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
IP
1/10 - 1/100.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
1/20.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
IP
1/10 - 1/100.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
1/20.

Target

  • Function

    CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.
  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P06729 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 914 Human
    • Omim: 186990 Human
    • SwissProt: P06729 Human
    • Unigene: 523500 Human
    • Alternative names

      • CD 2 antibody
      • CD2 antibody
      • CD2 antigen (p50), sheep red blood cell receptor antibody
      • CD2 antigen antibody
      • CD2 molecule antibody
      • CD2_HUMAN antibody
      • Erythrocyte receptor antibody
      • FLJ46032 antibody
      • LFA-2 antibody
      • LFA-3 receptor antibody
      • LFA2 antibody
      • LFA3 receptor antibody
      • Ly-37 antibody
      • Lymphocyte function antigen 2 antibody
      • lymphocyte-function antigen-2 antibody
      • OTTHUMP00000024366 antibody
      • Rosette receptor antibody
      • Sheep erythrocyte receptor antibody
      • SRBC antibody
      • T cell surface antigen CD2 antibody
      • T-cell surface antigen CD2 antibody
      • T-cell surface antigen T11/Leu-5 antibody
      • T-lymphocyte surface CD2 antigen antibody
      • T11 antibody
      see all

    Images

    • Flow Cytometry - Anti-CD2 antibody [EPR6451] (ab131276)
      Flow Cytometry - Anti-CD2 antibody [EPR6451] (ab131276)

      Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling CD2 with unpurified ab131276 at 1/20 dilution (red). The secondary antibody was Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

    • Western blot - Anti-CD2 antibody [EPR6451] (ab131276)
      Western blot - Anti-CD2 antibody [EPR6451] (ab131276)
      All lanes : Anti-CD2 antibody [EPR6451] (ab131276) at 1/1000 dilution

      Lane 1 : Human thymus lysate
      Lane 2 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 39 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)

      Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelled with ab131276 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)

      Immunohistochemical analysis of paraffin embedded Human colon tissue labelled with ab131276 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)

      ab131276 showing positive staining in Diffuse large B-cell lymphoma tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)

      ab131276 showing positive staining in Normal spleen tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR6451] (ab131276)

      ab131276 showing positive staining in Hodgkin's lymphoma tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-CD2 antibody [EPR6451] (ab131276)
      Anti-CD2 antibody [EPR6451] (ab131276)

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab131276? Please let us know so that we can cite the reference in this datasheet.

    ab131276 has been referenced in 2 publications.

    • Santucci-Pereira J  et al. Genomic signature of parity in the breast of premenopausal women. Breast Cancer Res 21:46 (2019). PubMed: 30922380
    • Companioni Nápoles O  et al. SCHLAFEN 5 expression correlates with intestinal metaplasia that progresses to gastric cancer. J Gastroenterol 52:39-49 (2017). PubMed: 27032393

    Customer reviews and Q&As

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