The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This antibody may be used with indirect labelling procedures on mononuclear cells separated on density gradient or on lysed whole blood.
CD2 immunofluorescence analysis can be performed on a flow cytometer equipped with an excitation source of 488nm and fitted with logarithmic amplifiers.
10µl of this antibody is sufficient for labelling of 1x106 cells.
CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.
Beyers AD et al. Activation of T lymphocytes via monoclonal antibodies against rat cell surface antigens with particular reference to CD2 antigen. Immunol Rev111:59-77 (1989).
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