The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Boil tissue sections in 1mM EDTA, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.
Pre-diluted / ready to use
CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.