Anti-CD2 antibody [TS218] - BSA and Azide free (ab175287)
Key features and details
- Mouse monoclonal [TS218] to CD2 - BSA and Azide free
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD2 antibody [TS218] - BSA and Azide free
See all CD2 primary antibodies -
Description
Mouse monoclonal [TS218] to CD2 - BSA and Azide free -
Host species
Mouse -
Specificity
Does not work with Rat in Western Blot applications -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein corresponding to Human CD2. Native protein
Database link: P06729 -
Positive control
- Human PBMC cells; Jurkat cells, Ramos, A-431, Raji.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified IgG from mouse ascites fluid. -
Clonality
Monoclonal -
Clone number
TS218 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175287 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 39 kDa.
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 39 kDa. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 914 Human
- Omim: 186990 Human
- SwissProt: P06729 Human
- Unigene: 523500 Human
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Alternative names
- CD 2 antibody
- CD2 antibody
- CD2 antigen (p50), sheep red blood cell receptor antibody
see all
Images
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All lanes : Anti-CD2 antibody [TS218] - BSA and Azide free (ab175287) at 2 µg/ml
Lane 1 : Ramos
Lane 2 : A-431
Lane 3 : K562
Lane 4 : Raji
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution
Predicted band size: 39 kDa -
Flow cytometric analysis of Jurkat cells labeling CD2 with ab175287 at a dilution of 0.5 µg/test (green), compared to an isotype control (blue). A Dylight 488-conjugated goat anti-mouse IgG (H+L) was used as the secondary antibody.
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Flow cytometric analysis of PBMC cells labeling CD2 with ab175287 at a dilution of 0.5 µg/test (green), compared to an isotype control (blue). A Dylight 488-conjugated goat anti-mouse IgG (H+L) was used as the secondary antibody.
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Flow cytometric analysis of PBMC cells labeling CD2 with ab175287 at a dilution of 0.5 µg/test (green), compared to an isotype control (blue). A Dylight 488-conjugated goat anti-mouse IgG (H+L) was used as the secondary antibody.
Datasheets and documents
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Datasheet download
References (0)
ab175287 has not yet been referenced specifically in any publications.