• Product name
    Anti-CD20 antibody [2H7]
    See all CD20 primary antibodies
  • Description
    Mouse monoclonal [2H7] to CD20
  • Host species
  • Tested applications
    Suitable for: IP, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Human, Non human primates
  • Immunogen

    Human tonsillar B cells

  • Epitope
    The epitope recognised by this antibody has been mapped to the following sequence which is found in the large extracellular loop of human CD20: YNCEPANPSEKNSPST



Our Abpromise guarantee covers the use of ab111274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use a concentration of 0.6 µg/ml.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.



  • Function
    This protein may be involved in the regulation of B-cell activation and proliferation.
  • Tissue specificity
    Expressed on B-cells.
  • Involvement in disease
    Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
  • Sequence similarities
    Belongs to the MS4A family.
  • Post-translational
    Phosphorylated. Might be functionally regulated by protein kinase(s).
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • APY antibody
    • ATOPY antibody
    • B lymphocyte antigen CD20 antibody
    • B Lymphocyte Cell Surface Antigen B1 antibody
    • B-lymphocyte antigen CD20 antibody
    • B-lymphocyte cell-surface antigen B1 antibody
    • B-lymphocyte surface antigen B1 antibody
    • B1 antibody
    • Bp 35 antibody
    • Bp35 antibody
    • CD 20 antibody
    • CD20 antibody
    • CD20 antigen antibody
    • CD20 receptor antibody
    • CD20_HUMAN antibody
    • CVID5 antibody
    • Fc epsilon receptor I beta chain antibody
    • Fc Fragment of IgE high affinity I receptor for beta polypeptide antibody
    • FCER1B antibody
    • High affinity immunoglobulin epsilon receptor subunit beta antibody
    • IgE Fc receptor subunit beta antibody
    • IGEL antibody
    • IGER antibody
    • IGHER antibody
    • LEU 16 antibody
    • LEU16 antibody
    • leukocyte surface antigen Leu 16 antibody
    • Leukocyte surface antigen Leu-16 antibody
    • Ly44 antibody
    • Membrane spanning 4 domains A1 antibody
    • Membrane spanning 4 domains subfamily A member 2 antibody
    • Membrane-spanning 4-domains subfamily A member 1 antibody
    • MGC3969 antibody
    • MS4A1 antibody
    • MS4A2 antibody
    • S7 antibody
    see all


ab111274 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Immunohistochemistry free floating
Human Tissue sections (Human B cells Lymphoma xenografted in rat tissue)
Human B cells Lymphoma xenografted in rat tissue

Fabien Fillesoye

Verified customer

Submitted Oct 21 2016


The 2H7 clone is not the same as the rituxan antibody and does not recognize the exact same epitope. I found an article that discusses in detail the differences between the two antibodies.

Crystal structure of chimeric antibody C2H7 Fab in complex with a CD20 peptide.
Mol Immunol. 2008 May;45(10):2861-8.

The following review discusses 2H7 (also known as C2H7) and several other CD20 monoclonal antibodies and how they differ from rituxan and other therapeutic anti-CD20 antibodies.

Epitope interactions of monoclonal antibodies targeting CD20 and their relationship to functional properties
MAbs. 2013 Jan 1; 5(1): 22–33.

The review includes this summary of some of the data from the first paper:

The CDR loops of 2H7 are structurally similar to those of rituximab. Among the four CDR loops that interact with CD20, only one (H3) differs substantially from the rituximab counterpart in terms of residue sequence and conformation.

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Thank you for contacting us.

Unfortunately we have not performed any kind of cytotoxicity assays with these antibodies. I was however able to find a paper referring to these assays with a murine monoclonal antibody anti CD20 from clone [B-Ly1] similar to our ab25607:



I hope this article is helpful. In any case, please notice that in order to perform functional applications azide-free antibodies would be required.

I am sorry for the lack of information and wish I could be more of help. Please do not hesitate to contact us for further assistance.

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