Overview

  • Product name
  • Description
    Rabbit polyclonal to CD20
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human CD20.
    Database link: P11836

  • Positive control
    • HEK293T, RAW264.7 and H9C2 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab201101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 33 kDa.

Target

  • Function
    This protein may be involved in the regulation of B-cell activation and proliferation.
  • Tissue specificity
    Expressed on B-cells.
  • Involvement in disease
    Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
  • Sequence similarities
    Belongs to the MS4A family.
  • Post-translational
    modifications
    Phosphorylated. Might be functionally regulated by protein kinase(s).
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • APY antibody
    • ATOPY antibody
    • B lymphocyte antigen CD20 antibody
    • B Lymphocyte Cell Surface Antigen B1 antibody
    • B-lymphocyte antigen CD20 antibody
    • B-lymphocyte cell-surface antigen B1 antibody
    • B-lymphocyte surface antigen B1 antibody
    • B1 antibody
    • Bp 35 antibody
    • Bp35 antibody
    • CD 20 antibody
    • CD20 antibody
    • CD20 antigen antibody
    • CD20 receptor antibody
    • CD20_HUMAN antibody
    • CVID5 antibody
    • Fc epsilon receptor I beta chain antibody
    • Fc Fragment of IgE high affinity I receptor for beta polypeptide antibody
    • FCER1B antibody
    • High affinity immunoglobulin epsilon receptor subunit beta antibody
    • IgE Fc receptor subunit beta antibody
    • IGEL antibody
    • IGER antibody
    • IGHER antibody
    • LEU 16 antibody
    • LEU16 antibody
    • leukocyte surface antigen Leu 16 antibody
    • Leukocyte surface antigen Leu-16 antibody
    • Ly44 antibody
    • Membrane spanning 4 domains A1 antibody
    • Membrane spanning 4 domains subfamily A member 2 antibody
    • Membrane-spanning 4-domains subfamily A member 1 antibody
    • MGC3969 antibody
    • MS4A1 antibody
    • MS4A2 antibody
    • S7 antibody
    see all

Images

  • All lanes : Anti-CD20 antibody (ab201101)

    Lane 1 : HEK293T whole cell lysate
    Lane 2 : RAW264.7 whole cell lysate
    Lane 3 : H9C2 whole cell lysate

    Predicted band size: 33 kDa

References

ab201101 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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1-3 of 3 Abreviews

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Hepa cell-bearing tumor)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
Hepa cell-bearing tumor
Blocking step
Hydrogen peroxide as blocking agent for 5 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 13 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (spleen)
Permeabilization
No
Specification
spleen
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 11 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6.0
Permeabilization
No
Specification
Tonsil
Blocking step
Hydrogen peroxide as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 27°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Sep 21 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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