Anti-CD20 antibody (ab244336)
Key features and details
- Rabbit polyclonal to CD20
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CD20 antibody
See all CD20 primary antibodies -
Description
Rabbit polyclonal to CD20 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human CD20 aa 200 to the C-terminus.
Database link: P11836 -
Positive control
- WB: Daudi cells ICC/IF: REH cells. IHC-P: Human gastrointestinal, tonsil, lymph node and spleen tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab244336 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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Notes |
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IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
Target
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Function
This protein may be involved in the regulation of B-cell activation and proliferation. -
Tissue specificity
Expressed on B-cells. -
Involvement in disease
Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
Sequence similarities
Belongs to the MS4A family. -
Post-translational
modificationsPhosphorylated. Might be functionally regulated by protein kinase(s). -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 931 Human
- Omim: 112210 Human
- SwissProt: P11836 Human
- Unigene: 712553 Human
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Alternative names
- APY antibody
- ATOPY antibody
- B lymphocyte antigen CD20 antibody
see all
Images
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All lanes : Anti-CD20 antibody (ab244336) at 0.4 µg/ml
Lane 1 : Daudi cells
Lane 2 : U-2 OS cells -
PFA fixed, Triton X-100 permeabilized REH (Human cell line) cells labeling CD20 using ab244336 at 4 µg/ml (green) in ICC/IF.
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Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD20 with ab244336 at a 1/1000 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human lymph node tissue stained for CD20 with ab244336 at a 1/1000 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human spleen tissue stained for CD20 with ab244336 at a 1/1000 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human gastrointestinal tissue stained for CD20 with ab244336 at a 1/1000 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human testis tissue stained for CD20 with ab244336 at a 1/1000 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for CD20 with ab244336 at a 1/1000 dilution in immunohistochemical analysis.
Low expression as expected.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab244336 has not yet been referenced specifically in any publications.