Product nameAnti-CD20 antibody
See all CD20 primary antibodies
DescriptionRabbit polyclonal to CD20
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Rat
- This antibody gave a positive signal in the following whole cell lysates: Raji; Ramos.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab85809 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).|
|IHC-P||Use a concentration of 5 µg/ml.|
FunctionThis protein may be involved in the regulation of B-cell activation and proliferation.
Tissue specificityExpressed on B-cells.
Involvement in diseaseDefects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
Sequence similaritiesBelongs to the MS4A family.
modificationsPhosphorylated. Might be functionally regulated by protein kinase(s).
- Information by UniProt
- APY antibody
- ATOPY antibody
- B lymphocyte antigen CD20 antibody
All lanes : Anti-CD20 antibody (ab85809) at 1 µg/ml
Lane 1 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Exposure time: 20 minutes
Human B-lymphocyte antigen CD20 contains a potential phosphorylation site (SwissProt) which may explain the banding pattern observed.
ab85809 has not yet been referenced specifically in any publications.