• Product name
  • Description
    Mouse polyclonal to CD20
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    CD20 (NP_068769.2, 1 a.a. ~ 297 a.a) full-length human protein.

  • Positive control
    • Human spleen membrane lysate Transfected 293T membrane lysate Human lymph node tissue CD20 expressing 293 cells



Our Abpromise guarantee covers the use of ab88247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use at an assay dependent concentration.

ab37355 - Mouse polyclonal IgG, is suitable for use as an isotype control with this antibody.



  • Function
    This protein may be involved in the regulation of B-cell activation and proliferation.
  • Tissue specificity
    Expressed on B-cells.
  • Involvement in disease
    Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
  • Sequence similarities
    Belongs to the MS4A family.
  • Post-translational
    Phosphorylated. Might be functionally regulated by protein kinase(s).
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • APY antibody
    • ATOPY antibody
    • B lymphocyte antigen CD20 antibody
    • B Lymphocyte Cell Surface Antigen B1 antibody
    • B-lymphocyte antigen CD20 antibody
    • B-lymphocyte cell-surface antigen B1 antibody
    • B-lymphocyte surface antigen B1 antibody
    • B1 antibody
    • Bp 35 antibody
    • Bp35 antibody
    • CD 20 antibody
    • CD20 antibody
    • CD20 antigen antibody
    • CD20 receptor antibody
    • CD20_HUMAN antibody
    • CVID5 antibody
    • Fc epsilon receptor I beta chain antibody
    • Fc Fragment of IgE high affinity I receptor for beta polypeptide antibody
    • FCER1B antibody
    • High affinity immunoglobulin epsilon receptor subunit beta antibody
    • IgE Fc receptor subunit beta antibody
    • IGEL antibody
    • IGER antibody
    • IGHER antibody
    • LEU 16 antibody
    • LEU16 antibody
    • leukocyte surface antigen Leu 16 antibody
    • Leukocyte surface antigen Leu-16 antibody
    • Ly44 antibody
    • Membrane spanning 4 domains A1 antibody
    • Membrane spanning 4 domains subfamily A member 2 antibody
    • Membrane-spanning 4-domains subfamily A member 1 antibody
    • MGC3969 antibody
    • MS4A1 antibody
    • MS4A2 antibody
    • S7 antibody
    see all


  • ab88247, at 3µg/ml, staining human CD20 in lymph node, using Immunohistochemistry, Formalin/PFA-fixed paraffin embedded tissue.
  • Anti-CD20 antibody (ab88247) at 1 µg/ml + Human spleen at 25 µg

    Goat Anti-Mouse IgG at 1/2500 dilution

    Observed band size: 33 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-CD20 antibody (ab88247) at 1 µg/ml

    Lane 1 : CD20 transfected lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    All lanes : Goat Anti-Mouse IgG at 1/2500 dilution

    Observed band size: 33 kDa why is the actual band size different from the predicted?

  • FACS analysis of negative control 293 cells (Black) and CD20 expressing 293 cells (Green) using ab88247.


This product has been referenced in:
  • Hu J  et al. Morin inhibits proliferation and self-renewal of CD133+ melanoma cells by upregulating miR-216a. J Pharmacol Sci 136:114-120 (2018). WB . Read more (PubMed: 29496393) »
  • Scialdone A  et al. The HDAC inhibitor valproate induces a bivalent status of the CD20 promoter in CLL patients suggesting distinct epigenetic regulation of CD20 expression in CLL in vivo. Oncotarget 8:37409-37422 (2017). Read more (PubMed: 28445158) »
See all 3 Publications for this product

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