Anti-CD20 antibody [AT80] (FITC) (ab27856)


  • Product name
    Anti-CD20 antibody [AT80] (FITC)
    See all CD20 primary antibodies
  • Description
    Mouse monoclonal [AT80] to CD20 (FITC)
  • Host species
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    Recognizes the human CD20 cell surface antigen, a glycoprotein of 35-37kD. Although it has not been epitope mapped, we believe that it recognizes a region on the extracellular surface of CD20 because no permeabilization treatment is required prior to staining.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    NS-O cells transfected with human CD20.

  • Positive control
    • Human peripheral blood cells
  • General notes

    Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC).



Our Abpromise guarantee covers the use of ab27856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use 10µl of neat solution or dilute to a maximum of 1/10 to label 106 cells or 100µl whole blood.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      This protein may be involved in the regulation of B-cell activation and proliferation.
    • Tissue specificity
      Expressed on B-cells.
    • Involvement in disease
      Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
    • Sequence similarities
      Belongs to the MS4A family.
    • Post-translational
      Phosphorylated. Might be functionally regulated by protein kinase(s).
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • APY antibody
      • ATOPY antibody
      • B lymphocyte antigen CD20 antibody
      • B Lymphocyte Cell Surface Antigen B1 antibody
      • B-lymphocyte antigen CD20 antibody
      • B-lymphocyte cell-surface antigen B1 antibody
      • B-lymphocyte surface antigen B1 antibody
      • B1 antibody
      • Bp 35 antibody
      • Bp35 antibody
      • CD 20 antibody
      • CD20 antibody
      • CD20 antigen antibody
      • CD20 receptor antibody
      • CD20_HUMAN antibody
      • CVID5 antibody
      • Fc epsilon receptor I beta chain antibody
      • Fc Fragment of IgE high affinity I receptor for beta polypeptide antibody
      • FCER1B antibody
      • High affinity immunoglobulin epsilon receptor subunit beta antibody
      • IgE Fc receptor subunit beta antibody
      • IGEL antibody
      • IGER antibody
      • IGHER antibody
      • LEU 16 antibody
      • LEU16 antibody
      • leukocyte surface antigen Leu 16 antibody
      • Leukocyte surface antigen Leu-16 antibody
      • Ly44 antibody
      • Membrane spanning 4 domains A1 antibody
      • Membrane spanning 4 domains subfamily A member 2 antibody
      • Membrane-spanning 4-domains subfamily A member 1 antibody
      • MGC3969 antibody
      • MS4A1 antibody
      • MS4A2 antibody
      • S7 antibody
      see all



    ab27856 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    I am sorry about that mix up, I mistyped the abnumber. Thank you for bringing it to my attention.   Again, this type of assay has not been tested by our lab but I have spoken with the lab and can offer you the following advice. That being said, I wo...

    Read More

    Thank you for your inquiry about ab27856. I've spoken with the lab about this and received the following response: This product has not been tested for neutralization assays. We have only tested it for use with flow cytometry. I’m guessing you are in...

    Read More

    Thank you for your enquiry. Although ab27856 has not been epitope mapped, the originator believes that it recognizes a region on the extracellular surface of CD20 because no permeabilization treatment is required prior to staining. For ab1169, th...

    Read More


    Sign up