Anti-CD20 antibody - C-terminal (ab194970)
Key features and details
- Goat polyclonal to CD20 - C-terminal
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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Overview
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Product name
Anti-CD20 antibody - C-terminal
See all CD20 primary antibodies -
Description
Goat polyclonal to CD20 - C-terminal -
Host species
Goat -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human CD20 aa 250 to the C-terminus (C terminal).
Database link: P11836 -
Positive control
- Human spleen tissue; Human tonsil tissue; Daudi cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab194970 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (3) |
Use a concentration of 2 - 2.5 µg/ml.
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WB |
Use a concentration of 3 - 5 µg/ml. Predicted molecular weight: 33 kDa.
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Notes |
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IHC-P
Use a concentration of 2 - 2.5 µg/ml. |
WB
Use a concentration of 3 - 5 µg/ml. Predicted molecular weight: 33 kDa. |
Target
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Function
This protein may be involved in the regulation of B-cell activation and proliferation. -
Tissue specificity
Expressed on B-cells. -
Involvement in disease
Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
Sequence similarities
Belongs to the MS4A family. -
Post-translational
modificationsPhosphorylated. Might be functionally regulated by protein kinase(s). -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 931 Human
- Omim: 112210 Human
- SwissProt: P11836 Human
- Unigene: 712553 Human
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Alternative names
- APY antibody
- ATOPY antibody
- B lymphocyte antigen CD20 antibody
see all
Images
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Anti-CD20 antibody - C-terminal (ab194970) at 4 µg/ml + Daudi lysate at 20 µg
Developed using the ECL technique.
Predicted band size: 33 kDaPrimary was incubated for 12 hours. Cell lysate prepared in RIPA buffer.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD20 with ab194970 at 2.5 μg/mL.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human spleen tissue labeling CD20 with ab194970 at 2.5 μg/mL.
Protocols
Datasheets and documents
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Datasheet download
References (4)
ab194970 has been referenced in 4 publications.
- Babst N et al. CXCR4, CXCR5 and CD44 May Be Involved in Homing of Lymphoma Cells into the Eye in a Patient Derived Xenograft Homing Mouse Model for Primary Vitreoretinal Lymphoma. Int J Mol Sci 23:N/A (2022). PubMed: 36233057
- Feng H et al. Transferrin Receptor Is a Specific Ferroptosis Marker. Cell Rep 30:3411-3423.e7 (2020). PubMed: 32160546
- Zhou X et al. The Role of BAFF-R Signaling in the Growth of Primary Central Nervous System Lymphoma. Front Oncol 10:682 (2020). PubMed: 32528875
- Serra-Peinado C et al. Expression of CD20 after viral reactivation renders HIV-reservoir cells susceptible to Rituximab. Nat Commun 10:3705 (2019). PubMed: 31420544