Anti-CD20 antibody [MEM-97] (ab8237)

Submit a review Q&A (2)References (3)
Flow Cytometry - Anti-CD20 antibody [MEM-97] (ab8237)
  • Immunoprecipitation - Anti-CD20 antibody [MEM-97] (ab8237)

Key features and details

  • Mouse monoclonal [MEM-97] to CD20
  • Suitable for: Flow Cyt, IP
  • Reacts with: Dog, Human, Pig
  • Isotype: IgG1

Overview

  • Product name

    Anti-CD20 antibody [MEM-97]
    See all CD20 primary antibodies

  • Description

    Mouse monoclonal [MEM-97] to CD20

  • Host species

    Mouse

  • Specificity

    CD20 antigen

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Dog
    Human
    Pig
    IP
    Human
    See all applications and species data

  • Immunogen

    Burkitt's lymphoma cell line Raji.

  • General notes

    This product was changed from ascites to tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab8237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Dog
Human
Pig
IP
Human
Application Abreviews Notes
Flow Cyt
Use a concentration of 10 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
IP
Use at an assay dependent concentration.
Notes
Flow Cyt
Use a concentration of 10 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
IP
Use at an assay dependent concentration.

Target

  • Function

    This protein may be involved in the regulation of B-cell activation and proliferation.

  • Tissue specificity

    Expressed on B-cells.

  • Involvement in disease

    Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.

  • Sequence similarities

    Belongs to the MS4A family.

  • Post-translational
    modifications

    Phosphorylated. Might be functionally regulated by protein kinase(s).

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • APY antibody
      • ATOPY antibody
      • B lymphocyte antigen CD20 antibody
      • B Lymphocyte Cell Surface Antigen B1 antibody
      • B-lymphocyte antigen CD20 antibody
      • B-lymphocyte cell-surface antigen B1 antibody
      • B-lymphocyte surface antigen B1 antibody
      • B1 antibody
      • Bp 35 antibody
      • Bp35 antibody
      • CD 20 antibody
      • CD20 antibody
      • CD20 antigen antibody
      • CD20 receptor antibody
      • CD20_HUMAN antibody
      • CVID 5 antibody
      • CVID5 antibody
      • Fc epsilon receptor I beta chain antibody
      • Fc Fragment of IgE high affinity I receptor for beta polypeptide antibody
      • FCER1B antibody
      • High affinity immunoglobulin epsilon receptor subunit beta antibody
      • IgE Fc receptor subunit beta antibody
      • IGEL antibody
      • IGER antibody
      • IGHER antibody
      • LEU 16 antibody
      • Leu-16 antibody
      • LEU16 antibody
      • leukocyte surface antigen Leu 16 antibody
      • Leukocyte surface antigen Leu-16 antibody
      • Ly44 antibody
      • Membrane spanning 4 domains A1 antibody
      • Membrane spanning 4 domains subfamily A member 2 antibody
      • membrane-spanning 4-domains A1 antibody
      • Membrane-spanning 4-domains subfamily A member 1 antibody
      • MGC3969 antibody
      • MS4A1 antibody
      • MS4A2 antibody
      • S7 antibody
      see all

    Images

    • Flow Cytometry - Anti-CD20 antibody [MEM-97] (ab8237)
      Flow Cytometry - Anti-CD20 antibody [MEM-97] (ab8237)

      Double Immunostaining of CD20 and CD79a in human, canine and porcine B lymphocytes using ab8237 and ab76908 by Flow Cytometery.

    • Immunoprecipitation - Anti-CD20 antibody [MEM-97] (ab8237)
      Immunoprecipitation - Anti-CD20 antibody [MEM-97] (ab8237)
      Immunoprecipitation of human CD20 from the whole cell lysate of RAJI human Burkitt lymphoma cell line. Western blot was immunostained with anti-CD20 rabbit polyclonal antibody. Lane 1: original lysate of RAJI cells Lane 2: immunoprecipitate by Isotypic mouse IgG1 control Lane 3: original lysate of RAJI cells Lane 4: immunoprecipitate by anti-CD20 (MEM-97, ab 8237)

    References (3)

    Publishing research using ab8237? Please let us know so that we can cite the reference in this datasheet.

    ab8237 has been referenced in 3 publications.

    • Zhang GP  et al. MicroRNA-98 regulates osteogenic differentiation of human bone mesenchymal stromal cells by targeting BMP2. J Cell Mol Med 21:254-264 (2017). Flow Cyt ; Human . PubMed: 27860183
    • Qiao Y  et al. A robust approach to enhance tumor-selective accumulation of nanoparticles. Oncotarget 2:59-68 (2011). Mouse . PubMed: 21378416
    • Szollosi J  et al. Supramolecular complexes of MHC class I, MHC class II, CD20, and tetraspan molecules (CD53, CD81, and CD82) at the surface of a B cell line JY. J Immunol 157:2939-46 (1996). PubMed: 8816400

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    Does Abcam offer custom conjugation of this antibody? Am looking for conjugation of MEM-97 to APC-Cy7 or another far red fluorochrome

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    Answer

    Thank you for your enquiry. I am sorry to confirm that we do not provide a customer conjugation service. We do have several conjugation kits available with different flourochromes. We have many available, including some for APC-Cy7 conjugation: ab102857 EasyLink APC/Cy7® Conjugation Kit (1x100µg APC/Cy7®) Click here (or use the following: https://www.abcam.com/EasyLink-APCCy7-Conjugation-Kit-1x100g-APCCy7-ab102857.html). ab102859 EasyLink APC/Cy7® Conjugation Kit (3 x 10µg APC/Cy7®) Click here (or use the following: https://www.abcam.com/APCCy7reg-Conjugation-Kit-Lightning-Linkreg-ab102859.html). There are many other conjugation kits which can be viewed on the following search results page from our website: https://www.abcam.com/index.html?pageconfig=searchresults&startPage=1 I hope this will be helpful to you. Should you have any further questions, please do not hesitate to contact us.

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    Question

    Dear Sir Please confirm if your product:ab8237/MEM-97 isotype IgG1 anti-CD20 is the same as mAb ibritumomab or if you have this refered antibody in your product list even with it's generic name.Please send me all necessary information of how to purchase for researchpurposes. It's important and urgent.! Thanks Sincerey Akin

    Read More
    Answer

    As far as we are aware they are not the same antibodies and we do not have a ibritumomab antibody in our catalogue. If you cannot find what you are looking for within the Abcam catalog, I suggest that you click on the link to "The World's Antibody Gateway". The World's Antibody Gateway is a free search engine service provided by Abcam to help you to quickly find the antibodies that you are looking for. It is a free-text search engine developed by the Abcam team so that it searches the catalogs of all online antibody companies (currently 249).

    Read More

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