Anti-CD20 antibody [MEM-97] (ab8237)
- Datasheet
- Specific References (3)
- Protocols
Overview
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Product nameAnti-CD20 antibody [MEM-97]
See all CD20 primary antibodies -
DescriptionMouse monoclonal [MEM-97] to CD20
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Host speciesMouse
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SpecificityCD20 antigen
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Tested applicationsSuitable for: Flow Cyt, IPmore details
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Species reactivityReacts with: Cow, Human, Pig
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Immunogen
Burkitt's lymphoma cell line Raji.
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General notes
This product was changed from ascites to tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityProtein A purified
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Purification notesPurified from TCS
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ClonalityMonoclonal
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Clone numberMEM-97
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IsotypeIgG1
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Light chain typeunknown
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab8237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| Flow Cyt | Use a concentration of 10 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| IP | Use at an assay dependent concentration. |
Target
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FunctionThis protein may be involved in the regulation of B-cell activation and proliferation.
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Tissue specificityExpressed on B-cells.
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Involvement in diseaseDefects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
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Sequence similaritiesBelongs to the MS4A family.
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Post-translational
modificationsPhosphorylated. Might be functionally regulated by protein kinase(s). -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 931 Human
- Omim: 112210 Human
- SwissProt: P11836 Human
- Unigene: 712553 Human
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Alternative names
- APY antibody
- ATOPY antibody
- B lymphocyte antigen CD20 antibody
see all
Images
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![Flow Cytometry - Anti-CD20 antibody [MEM-97] (ab8237)](https://www.abcam.com/ps/products/8/ab8237/Images/ab8237_1.jpg)
Flow Cytometry - Anti-CD20 antibody [MEM-97] (ab8237)Double Immunostaining of CD20 and CD79a in human, canine and porcine B lymphocytes using ab8237 and ab76908 by Flow Cytometery.
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![Immunoprecipitation - Anti-CD20 antibody [MEM-97] (ab8237)](https://www.abcam.com/ps/products/8/ab8237/Images/CD20-Primary-antibodies-ab8237-1.jpg)
Immunoprecipitation - Anti-CD20 antibody [MEM-97] (ab8237)Immunoprecipitation of human CD20 from the whole cell lysate of RAJI human Burkitt lymphoma cell line. Western blot was immunostained with anti-CD20 rabbit polyclonal antibody. Lane 1: original lysate of RAJI cells Lane 2: immunoprecipitate by Isotypic mouse IgG1 control Lane 3: original lysate of RAJI cells Lane 4: immunoprecipitate by anti-CD20 (MEM-97, ab 8237)
References
This product has been referenced in:
- Zhang GP et al. MicroRNA-98 regulates osteogenic differentiation of human bone mesenchymal stromal cells by targeting BMP2. J Cell Mol Med 21:254-264 (2017). Flow Cyt ; Human . Read more (PubMed: 27860183) »
- Qiao Y et al. A robust approach to enhance tumor-selective accumulation of nanoparticles. Oncotarget 2:59-68 (2011). Mouse . Read more (PubMed: 21378416) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"