Anti-CD20 antibody [MEM-97] (ab8237)
Key features and details
- Mouse monoclonal [MEM-97] to CD20
- Suitable for: Flow Cyt, IP
- Reacts with: Dog, Human, Pig
- Isotype: IgG1
Overview
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Product name
Anti-CD20 antibody [MEM-97]
See all CD20 primary antibodies -
Description
Mouse monoclonal [MEM-97] to CD20 -
Host species
Mouse -
Specificity
CD20 antigen -
Tested Applications & Species
Application Species Flow Cyt DogHumanPigIP Human -
Immunogen
Burkitt's lymphoma cell line Raji.
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General notes
This product was changed from ascites to tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
MEM-97 -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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Flow Cyt |
Dog
Human
Pig
|
IP |
Human
|
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use a concentration of 10 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
|
IP |
Use at an assay dependent concentration.
|
Notes |
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Flow Cyt
Use a concentration of 10 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
IP
Use at an assay dependent concentration. |
Target
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Function
This protein may be involved in the regulation of B-cell activation and proliferation. -
Tissue specificity
Expressed on B-cells. -
Involvement in disease
Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
Sequence similarities
Belongs to the MS4A family. -
Post-translational
modificationsPhosphorylated. Might be functionally regulated by protein kinase(s). -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 485430 Dog
- Entrez Gene: 931 Human
- Omim: 112210 Human
- SwissProt: Q3C2E2 Dog
- SwissProt: P11836 Human
- Unigene: 712553 Human
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Alternative names
- APY antibody
- ATOPY antibody
- B lymphocyte antigen CD20 antibody
see all
Images
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Double Immunostaining of CD20 and CD79a in human, canine and porcine B lymphocytes using ab8237 and ab76908 by Flow Cytometery.
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Immunoprecipitation of human CD20 from the whole cell lysate of RAJI human Burkitt lymphoma cell line. Western blot was immunostained with anti-CD20 rabbit polyclonal antibody. Lane 1: original lysate of RAJI cells Lane 2: immunoprecipitate by Isotypic mouse IgG1 control Lane 3: original lysate of RAJI cells Lane 4: immunoprecipitate by anti-CD20 (MEM-97, ab 8237)
Protocols
References (3)
ab8237 has been referenced in 3 publications.
- Zhang GP et al. MicroRNA-98 regulates osteogenic differentiation of human bone mesenchymal stromal cells by targeting BMP2. J Cell Mol Med 21:254-264 (2017). Flow Cyt ; Human . PubMed: 27860183
- Qiao Y et al. A robust approach to enhance tumor-selective accumulation of nanoparticles. Oncotarget 2:59-68 (2011). Mouse . PubMed: 21378416
- Szollosi J et al. Supramolecular complexes of MHC class I, MHC class II, CD20, and tetraspan molecules (CD53, CD81, and CD82) at the surface of a B cell line JY. J Immunol 157:2939-46 (1996). PubMed: 8816400