Recombinant Anti-CD20 antibody [rIGEL/773] (ab219329)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [rIGEL/773] to CD20
- Suitable for: Protein Array, IHC-P, ICC, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-CD20 antibody [rIGEL/773]
See all CD20 primary antibodies -
Description
Mouse monoclonal [rIGEL/773] to CD20 -
Host species
Mouse -
Tested applications
Suitable for: Protein Array, IHC-P, ICC, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human CD20 aa 1-297.
Database link: P11836 -
Positive control
- IHC-P: Human tonsil tissue; ICC and Flow Cyt: Raji cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
rIGEL/773 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab219329 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Protein Array |
Use at an assay dependent concentration.
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IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC |
Use a concentration of 1 - 2 µg/ml.
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Flow Cyt |
Use 1-2µg for 106 cells.
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Notes |
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Protein Array
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC
Use a concentration of 1 - 2 µg/ml. |
Flow Cyt
Use 1-2µg for 106 cells. |
Target
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Function
This protein may be involved in the regulation of B-cell activation and proliferation. -
Tissue specificity
Expressed on B-cells. -
Involvement in disease
Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
Sequence similarities
Belongs to the MS4A family. -
Post-translational
modificationsPhosphorylated. Might be functionally regulated by protein kinase(s). -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 931 Human
- Omim: 112210 Human
- SwissProt: P11836 Human
- Unigene: 712553 Human
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Alternative names
- APY antibody
- ATOPY antibody
- B lymphocyte antigen CD20 antibody
see all
Images
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Flow Cytometry analysis of Raji (human Burkitt's lymphoma cell line) cells labeling CD20 with ab219329 at 2 µg/106 cells followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
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Immunocytochemistry analysis of Raji (human Burkitt's lymphoma cell line) cells labeling CD20 with ab219329 at 2 µg/mL followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot (red).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [rIGEL/773] (ab219329)
Immunohistochemical analysis of formalin-fixed paraffin-embedded human tonsil tissue sections labeling CD20 with ab219329 at 1 µg/mL.
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ab219329 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab219329 has not yet been referenced specifically in any publications.