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  1. Link

    cd20-antibody-rigel773-ab219329.pdf

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Immunology Adaptive Immunity B Cells CD
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Recombinant

Recombinant Anti-CD20 antibody [rIGEL/773] (ab219329)

  • Datasheet
  • SDS
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Flow Cytometry - Anti-CD20 antibody [rIGEL/773] (ab219329)
  • Immunocytochemistry - Anti-CD20 antibody [rIGEL/773] (ab219329)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [rIGEL/773] (ab219329)
  • Protein Array - Anti-CD20 antibody [rIGEL/773] (ab219329)
  • Anti-CD20 antibody [rIGEL/773] (ab219329)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [rIGEL/773] to CD20
  • Suitable for: Protein Array, IHC-P, ICC, Flow Cyt
  • Reacts with: Human

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Overview

  • Product name

    Anti-CD20 antibody [rIGEL/773]
    See all CD20 primary antibodies
  • Description

    Mouse monoclonal [rIGEL/773] to CD20
  • Host species

    Mouse
  • Tested applications

    Suitable for: Protein Array, IHC-P, ICC, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human CD20 aa 1-297.
    Database link: P11836

  • Positive control

    • IHC-P: Human tonsil tissue; ICC and Flow Cyt: Raji cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 0.05% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    rIGEL/773
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Human Lineage Negative
    • Cancer
    • Tumor immunology
    • Tumor-associated antigens
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP kits
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Related Products

    • Recombinant Human CD20 protein (ab158047)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab219329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Protein Array
Use at an assay dependent concentration.
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC
Use a concentration of 1 - 2 µg/ml.
Flow Cyt
Use 1-2µg for 106 cells.
Notes
Protein Array
Use at an assay dependent concentration.
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC
Use a concentration of 1 - 2 µg/ml.
Flow Cyt
Use 1-2µg for 106 cells.

Target

  • Function

    This protein may be involved in the regulation of B-cell activation and proliferation.
  • Tissue specificity

    Expressed on B-cells.
  • Involvement in disease

    Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
  • Sequence similarities

    Belongs to the MS4A family.
  • Post-translational
    modifications

    Phosphorylated. Might be functionally regulated by protein kinase(s).
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P11836 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 931 Human
    • Omim: 112210 Human
    • SwissProt: P11836 Human
    • Unigene: 712553 Human
    • Alternative names

      • APY antibody
      • ATOPY antibody
      • B lymphocyte antigen CD20 antibody
      • B Lymphocyte Cell Surface Antigen B1 antibody
      • B-lymphocyte antigen CD20 antibody
      • B-lymphocyte cell-surface antigen B1 antibody
      • B-lymphocyte surface antigen B1 antibody
      • B1 antibody
      • Bp 35 antibody
      • Bp35 antibody
      • CD 20 antibody
      • CD20 antibody
      • CD20 antigen antibody
      • CD20 receptor antibody
      • CD20_HUMAN antibody
      • CVID 5 antibody
      • CVID5 antibody
      • Fc epsilon receptor I beta chain antibody
      • Fc Fragment of IgE high affinity I receptor for beta polypeptide antibody
      • FCER1B antibody
      • High affinity immunoglobulin epsilon receptor subunit beta antibody
      • IgE Fc receptor subunit beta antibody
      • IGEL antibody
      • IGER antibody
      • IGHER antibody
      • LEU 16 antibody
      • Leu-16 antibody
      • LEU16 antibody
      • leukocyte surface antigen Leu 16 antibody
      • Leukocyte surface antigen Leu-16 antibody
      • Ly44 antibody
      • Membrane spanning 4 domains A1 antibody
      • Membrane spanning 4 domains subfamily A member 2 antibody
      • membrane-spanning 4-domains A1 antibody
      • Membrane-spanning 4-domains subfamily A member 1 antibody
      • MGC3969 antibody
      • MS4A1 antibody
      • MS4A2 antibody
      • S7 antibody
      see all

    Images

    • Flow Cytometry - Anti-CD20 antibody [rIGEL/773] (ab219329)
      Flow Cytometry - Anti-CD20 antibody [rIGEL/773] (ab219329)

      Flow Cytometry analysis of Raji (human Burkitt's lymphoma cell line) cells labeling CD20 with ab219329 at 2 µg/106 cells followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

    • Immunocytochemistry - Anti-CD20 antibody [rIGEL/773] (ab219329)
      Immunocytochemistry - Anti-CD20 antibody [rIGEL/773] (ab219329)

      Immunocytochemistry analysis of Raji (human Burkitt's lymphoma cell line) cells labeling CD20 with ab219329 at 2 µg/mL followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot (red).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [rIGEL/773] (ab219329)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [rIGEL/773] (ab219329)

      Immunohistochemical analysis of formalin-fixed paraffin-embedded human tonsil tissue sections labeling CD20 with ab219329 at 1 µg/mL.

    • Protein Array - Anti-CD20 antibody [rIGEL/773] (ab219329)
      Protein Array - Anti-CD20 antibody [rIGEL/773] (ab219329)
      ab219329 was tested in protein array against over 19000 different full-length human proteins.
      Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
      A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
    • Anti-CD20 antibody [rIGEL/773] (ab219329)
      Anti-CD20 antibody [rIGEL/773] (ab219329)

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab219329? Please let us know so that we can cite the reference in this datasheet.

    ab219329 has not yet been referenced specifically in any publications.

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