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    cd20-antibody-sp32-ab64088.pdf

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Immunology Adaptive Immunity B Cells CD
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RecombinantRabMAb

Recombinant Anti-CD20 antibody [SP32] (ab64088)

  • Datasheet
  • SDS
Reviews (8) Submit a question References (3)

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Flow Cytometry - Anti-CD20 antibody [SP32] (ab64088)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] (ab64088)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD20 antibody [SP32] (ab64088)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] (ab64088)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] (ab64088)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] (ab64088)
  • Flow Cytometry - Anti-CD20 antibody [SP32] (ab64088)
  • Western blot - Anti-CD20 antibody [SP32] (ab64088)
  • Flow Cytometry - Anti-CD20 antibody [SP32] (ab64088)
  • Anti-CD20 antibody [SP32] (ab64088)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP32] to CD20
  • Suitable for: WB, Flow Cyt, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-CD20 antibody [SP32]
    See all CD20 primary antibodies
  • Description

    Rabbit monoclonal [SP32] to CD20
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptide within Human CD20 aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: P11836

  • Positive control

    • IHC-P: Human tonsil tissue. Mouse and rat spleen tissues. ICC: Ramos cells. Flow Cyt: WEHI-231 and Ramos cells. WB: Ramos cell lysate.
  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from TCS by protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    SP32
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Human Lineage Negative
    • Cancer
    • Tumor immunology
    • Tumor-associated antigens

Associated products

  • Alternative Versions

    • Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Ramos whole cell lysate (ab3955)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab64088 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
Use at an assay dependent concentration.
Flow Cyt
1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P (4)
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF (1)
1/100.
Notes
WB
Use at an assay dependent concentration.
Flow Cyt
1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100.

Target

  • Function

    This protein may be involved in the regulation of B-cell activation and proliferation.
  • Tissue specificity

    Expressed on B-cells.
  • Involvement in disease

    Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
  • Sequence similarities

    Belongs to the MS4A family.
  • Post-translational
    modifications

    Phosphorylated. Might be functionally regulated by protein kinase(s).
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P11836 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 485430 Dog
    • Entrez Gene: 931 Human
    • Entrez Gene: 12482 Mouse
    • Entrez Gene: 309217 Rat
    • Omim: 112210 Human
    • SwissProt: Q3C2E2 Dog
    • SwissProt: P11836 Human
    • SwissProt: P19437 Mouse
    • Unigene: 712553 Human
    • Unigene: 4046 Mouse
    see all
  • Alternative names

    • APY antibody
    • ATOPY antibody
    • B lymphocyte antigen CD20 antibody
    • B Lymphocyte Cell Surface Antigen B1 antibody
    • B-lymphocyte antigen CD20 antibody
    • B-lymphocyte cell-surface antigen B1 antibody
    • B-lymphocyte surface antigen B1 antibody
    • B1 antibody
    • Bp 35 antibody
    • Bp35 antibody
    • CD 20 antibody
    • CD20 antibody
    • CD20 antigen antibody
    • CD20 receptor antibody
    • CD20_HUMAN antibody
    • CVID 5 antibody
    • CVID5 antibody
    • Fc epsilon receptor I beta chain antibody
    • Fc Fragment of IgE high affinity I receptor for beta polypeptide antibody
    • FCER1B antibody
    • High affinity immunoglobulin epsilon receptor subunit beta antibody
    • IgE Fc receptor subunit beta antibody
    • IGEL antibody
    • IGER antibody
    • IGHER antibody
    • LEU 16 antibody
    • Leu-16 antibody
    • LEU16 antibody
    • leukocyte surface antigen Leu 16 antibody
    • Leukocyte surface antigen Leu-16 antibody
    • Ly44 antibody
    • Membrane spanning 4 domains A1 antibody
    • Membrane spanning 4 domains subfamily A member 2 antibody
    • membrane-spanning 4-domains A1 antibody
    • Membrane-spanning 4-domains subfamily A member 1 antibody
    • MGC3969 antibody
    • MS4A1 antibody
    • MS4A2 antibody
    • S7 antibody
    see all

Images

  • Flow Cytometry - Anti-CD20 antibody [SP32] (ab64088)
    Flow Cytometry - Anti-CD20 antibody [SP32] (ab64088)

    Flow cytometry analysis of Ramos (human Burkitt's lymphoma) labeling CD20 with purified ab64088 at 1/40 dilution (7.78 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) (Black). Unlableled control -Unlabelled cells (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] (ab64088)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] (ab64088)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling CD20 with ab64088 at 1/100 dilution (3.11 µg/ml). Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on rat spleen, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab64088 for 30 mins at room temperature

  • Immunocytochemistry/ Immunofluorescence - Anti-CD20 antibody [SP32] (ab64088)
    Immunocytochemistry/ Immunofluorescence - Anti-CD20 antibody [SP32] (ab64088)

    Immunocytochemistry/ Immunofluorescence analysis of Ramos (human Burkitt's lymphoma B lymphocyte) cells labeling CD20 with purified ab64088 at 1/100 (3.1 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] (ab64088)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] (ab64088)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse spleen tissue sections labeling CD20 with ab64088 at 1/100 dilution (3.11 µg/ml). Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on mouse spleen, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab64088 for 30 mins at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] (ab64088)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] (ab64088)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD20 with ab64088 at 1/100 dilution (3.11 µg/ml). Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab64088 for 30 mins at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] (ab64088)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] (ab64088)
    Immunohistochemical analysis of CD20 expression in formalin fixed, paraffin embedded human tonsil tissue using ab64088 at a 1/100 dilution.
  • Flow Cytometry - Anti-CD20 antibody [SP32] (ab64088)
    Flow Cytometry - Anti-CD20 antibody [SP32] (ab64088)

    Flow cytometry analysis of WEHI-231 (mouse lymphoblast B cell lymphoma) labeling CD20 with purified ab64088 at 1/40 dilution (7.78 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) (Black). Unlableled control -Unlabelled cells (blue).

  • Western blot - Anti-CD20 antibody [SP32] (ab64088)
    Western blot - Anti-CD20 antibody [SP32] (ab64088)
    Anti-CD20 antibody [SP32] (ab64088) at 1/100 dilution + lysate prepared from Ramos cells

    Observed band size: 33 kDa why is the actual band size different from the predicted?

  • Flow Cytometry - Anti-CD20 antibody [SP32] (ab64088)
    Flow Cytometry - Anti-CD20 antibody [SP32] (ab64088)

    Flow cytometric analysis of rabbit anti-CD20 (SP32) antibody ab64088(1/100)  in Jurkat cells (green) compared to negative control of rabbit IgG (blue).

  • Anti-CD20 antibody [SP32] (ab64088)
    Anti-CD20 antibody [SP32] (ab64088)

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (3)

Publishing research using ab64088? Please let us know so that we can cite the reference in this datasheet.

ab64088 has been referenced in 3 publications.

  • Tam A  et al. Sex differences in lymphoid follicles in COPD airways. Respir Res 21:46 (2020). PubMed: 32033623
  • Green KY  et al. Human norovirus targets enteroendocrine epithelial cells in the small intestine. Nat Commun 11:2759 (2020). PubMed: 32488028
  • Shinojima N  et al. Clinical significance of polyglutamylation in primary central nervous system lymphoma. Acta Neuropathol Commun 6:15 (2018). PubMed: 29475458

Customer reviews and Q&As

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1-8 of 8 Abreviews

Immunocytochemistry/ Immunofluorescence abreview for Anti-CD20 antibody [SP32]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (PBMCs)
Permeabilization
Yes - Triton X-100 in PBS
Specification
PBMCs
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 21°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Jan 21 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD20 antibody [SP32]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lymphoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA
Permeabilization
No
Specification
Lymphoma
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: RT°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Jan 14 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD20 antibody [SP32]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (palatine tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Specification
palatine tonsil
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Jan 11 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD20 antibody [SP32]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Specification
spleen
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Jan 11 2021

Immunohistochemistry (Frozen sections) abreview for Anti-CD20 antibody [SP32]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (spleen)
Permeabilization
No
Specification
spleen
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Acetone
Read More

Abcam user community

Verified customer

Submitted Jan 11 2021

Immunohistochemistry (Frozen sections) abreview for Anti-CD20 antibody [SP32]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (B cell lymphoma)
Permeabilization
No
Specification
B cell lymphoma
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 21°C
Fixative
Acetone
Read More

Abcam user community

Verified customer

Submitted Dec 21 2020

Western blot abreview for Anti-CD20 antibody [SP32]

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Dog Cell lysate - whole cell (MDCK)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Acrylamide)
Loading amount
45 µg
Specification
MDCK
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More

Dr. Tony Durand

Verified customer

Submitted Jul 10 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD20 antibody [SP32]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Bone marrow)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
Bone marrow
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Fixative
Formaldehyde
Read More

Dr. Queralt Vallmajó

Verified customer

Submitted Mar 18 2020

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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