Recombinant Anti-CD200 / OX2 antibody [EPR22412-53] - BSA and Azide free (ab254213)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22412-53] to CD200 / OX2 - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD200 / OX2 antibody [EPR22412-53] - BSA and Azide free
See all CD200 / OX2 primary antibodies -
Description
Rabbit monoclonal [EPR22412-53] to CD200 / OX2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or WB -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: Human peripheral blood mononuclear cells.
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General notes
ab254213 is the carrier-free version of ab252920.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22412-53 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab254213 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
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Notes |
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Flow Cyt
Use at an assay dependent concentration. |
Target
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Function
Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 4345 Human
- Omim: 155970 Human
- SwissProt: P41217 Human
- Unigene: 79015 Human
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Alternative names
- Antigen identified by monoclonal antibody MRC OX 2 antibody
- CD200 antibody
- CD200 antigen antibody
see all
Images
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Flow cytometric analysis of 4% paraformaldehyde-fixed human peripheral blood mononuclear cells labeling CD200 at 1/50 dilution (right) compared to a Rabbit monoclonal IgG (ab172730, Left) isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, ab150097) was used as the secondary antibody at 1/5000 dilution.
Cells were stained with rabbit IgG (Left) or ab252920 (Right). Then stained with anti-CD19 conjugated with Alexa Fluor® 647. The expression pattern is consistent with literature (PMID: 28587354).
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252920).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab254213 has not yet been referenced specifically in any publications.