Overview

  • Product name

    Anti-CD200 / OX2 antibody [MRC OX-2]
    See all CD200 / OX2 primary antibodies
  • Description

    Mouse monoclonal [MRC OX-2] to CD200 / OX2
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Rat
  • Immunogen

    Tissue, cells or virus corresponding to Rat CD200/ OX2.

  • Positive control

    • Flow Cyt: Lewis rat splenocytes.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from TCS.
  • Primary antibody notes

    This antibody is a useful marker for the follicular dendritic cells in lymphoid tissues as it does not label the dendritic (interdigitating) cells in T areas that are Ia antigen positive.
  • Clonality

    Monoclonal
  • Clone number

    MRC OX-2
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab22353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 0.2 µg/ml.

Target

  • Function

    Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues.
  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Antigen identified by monoclonal antibody MRC OX 2 antibody
    • CD200 antibody
    • CD200 antigen antibody
    • CD200 molecule antibody
    • MOX1 antibody
    • MOX2 antibody
    • MRC antibody
    • MRC OX 2 antigen antibody
    • My033 antibody
    • OX 2 antibody
    • OX 2 membrane glycoprotein precursor antibody
    • OX-2 membrane glycoprotein antibody
    • OX2G antibody
    • OX2G_HUMAN antibody
    see all

Images

  • Lewis rat splenocytes stained with ab22353 (right) or mouse IgG1κ (left). Lewis rat splenocytes were incubated for 30 min on ice in 10% rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab22353) or mouse IgG1κ Isotype (ab170190) (1x106 in 100µl at 0.2 µg/ml) for 30 min on ice.

    The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 APC antibody.

    Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.

     

References

ab22353 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question
Answer

Thank you for your enquiry. In the process of setting up this return for you I noticed that your colleage who had already called our customer service department and arranged to ship this product back to Abcam.  I do hope everything is taken care of. If you have any further questions about this return feel free to contact me.

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