Anti-CD200 / OX2 antibody [MRC OX-2] - BSA and Azide free (ab244560)
![Flow Cytometry - Anti-CD200 / OX2 antibody [MRC OX-2] - BSA and Azide free (ab244560)](https://www.abcam.com/ps/products/244/ab244560/Images/ab244560-346727-AntiCD200--OX2-antibody-MRC-OX2-flow-cytometry-lewis-rat-splenocyte.jpg "Flow Cytometry - Anti-CD200 / OX2 antibody [MRC OX-2] - BSA and Azide free (ab244560)")
Key features and details
- Mouse monoclonal [MRC OX-2] to CD200 / OX2 - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Rat
- Isotype: IgG1
Overview
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Product name
Anti-CD200 / OX2 antibody [MRC OX-2] - BSA and Azide free
See all CD200 / OX2 primary antibodies -
Description
Mouse monoclonal [MRC OX-2] to CD200 / OX2 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Tissue, cells or virus corresponding to Rat CD200/ OX2. Rat thymocyte membrane glycoprotein.
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Positive control
- Flow Cyt: Lewis rat splenocytes.
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General notes
ab244560 is a PBS-only buffer format of ab22353. Please refer to ab22353 for recommended dilutions, protocols, and image data.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
MRC OX-2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
Applications
Our Abpromise guarantee covers the use of ab244560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use a concentration of 0.2 µg/ml. |
Target
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Function
Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 24560 Rat
- SwissProt: P04218 Rat
- Unigene: 7085 Rat
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Alternative names
- Antigen identified by monoclonal antibody MRC OX 2 antibody
- CD200 antibody
- CD200 antigen antibody
see all
Images
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Flow Cytometry - Anti-CD200 / OX2 antibody [MRC OX-2] - BSA and Azide free (ab244560)
This data was generating using the same clone in a different formulation (ab22353).
Lewis rat splenocytes stained with ab22353 (right) or mouse IgG1κ (left). Lewis rat splenocytes were incubated for 30 min on ice in 10% rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab22353) or mouse IgG1κ Isotype (ab170190) (1x106 in 100µl at 0.2 µg/ml) for 30 min on ice.
The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 APC antibody.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab244560 has not yet been referenced specifically in any publications.
