Anti-CD200 / OX2 antibody [MRC OX-2] - BSA and Azide free (ab244560)
![Flow Cytometry - Anti-CD200 / OX2 antibody [MRC OX-2] - BSA and Azide free (ab244560)](https://www.abcam.com/ps/products/244/ab244560/Images/ab244560-346727-AntiCD200--OX2-antibody-MRC-OX2-flow-cytometry-lewis-rat-splenocyte.jpg "Flow Cytometry - Anti-CD200 / OX2 antibody [MRC OX-2] - BSA and Azide free (ab244560)")
Key features and details
- Mouse monoclonal [MRC OX-2] to CD200 / OX2 - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Rat
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-CD200 / OX2 antibody [MRC OX-2] - BSA and Azide free
See all CD200 / OX2 primary antibodies -
Description
Mouse monoclonal [MRC OX-2] to CD200 / OX2 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Tissue, cells or virus corresponding to Rat CD200/ OX2. Rat thymocyte membrane glycoprotein.
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Positive control
- Flow Cyt: Lewis rat splenocytes.
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General notes
ab244560 is the carrier-free version of ab22353.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
MRC OX-2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab244560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use a concentration of 0.2 µg/ml.
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| Notes |
|---|
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Flow Cyt
Use a concentration of 0.2 µg/ml. |
Target
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Function
Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 24560 Rat
- SwissProt: P04218 Rat
- Unigene: 7085 Rat
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Alternative names
- Antigen identified by monoclonal antibody MRC OX 2 antibody
- CD200 antibody
- CD200 antigen antibody
see all
Images
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Flow Cytometry - Anti-CD200 / OX2 antibody [MRC OX-2] - BSA and Azide free (ab244560)
This data was generating using the same clone in a different formulation (ab22353).
Lewis rat splenocytes stained with ab22353 (right) or mouse IgG1κ (left). Lewis rat splenocytes were incubated for 30 min on ice in 10% rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab22353) or mouse IgG1κ Isotype (ab170190) (1x106 in 100µl at 0.2 µg/ml) for 30 min on ice.
The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 APC antibody.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab244560 has not yet been referenced specifically in any publications.
