Key features and details
- Rat monoclonal [MRC OX90] to CD200 / OX2 - BSA and Azide free
- Suitable for: IHC-Fr, IHC-FoFr, Flow Cyt, ELISA
- Reacts with: Mouse
- Isotype: IgG2a
Product nameAnti-CD200 / OX2 antibody [MRC OX90] - BSA and Azide free
See all CD200 / OX2 primary antibodies
DescriptionRat monoclonal [MRC OX90] to CD200 / OX2 - BSA and Azide free
Tested applicationsSuitable for: IHC-Fr, IHC-FoFr, Flow Cyt, ELISAmore details
Species reactivityReacts with: Mouse
Fusion protein corresponding to CD200/ OX2. Mouse CD200-rat CD4 fusion protein.
- Flow Cyt: C57BL/6 splenocytes.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies.
For details on our patents, please refer to www.abcam.com/RabMAbPatents
Spleen cells from immunised rats were fused with cells of the rat Y3 myeloma cell line.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact email@example.com.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurified from TCS.
Clone numberMRC OX90
Light chain typekappa
Our Abpromise guarantee covers the use of ab244564 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration.|
|Flow Cyt||Use a concentration of 0.2 µg/ml.|
|ELISA||Use at an assay dependent concentration.|
FunctionCostimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues.
Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationCell membrane.
- Information by UniProt
- Antigen identified by monoclonal antibody MRC OX 2 antibody
- CD200 antibody
- CD200 antigen antibody
This data was developed using the same antibody clone in a different buffer formulation (ab33734)
C57BL/6 mouse splenocytes stained with ab33734 (right) or Rat IgG2aκ (ab18450) isotype (left). C57BL/6 mouse splenocytes were incubated for 30 min on ice in 1x PBS / 10 % mouse serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab33734) or Rat IgG2aκ (ab18450) (1x106 in 100 µl at 0.2 µg/ml) for 30 min on ice.
The secondary antibody Goat anti-rat IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150165) was used at 1/2000 dilution for 30 min on ice. The cells were simultaneously stained with CD19 antibody.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab244564 has not yet been referenced specifically in any publications.