Anti-CD200 / OX2 antibody [MRC OX90] - BSA and Azide free (ab244564)

Overview

  • Product name

    Anti-CD200 / OX2 antibody [MRC OX90] - BSA and Azide free
    See all CD200 / OX2 primary antibodies
  • Description

    Rat monoclonal [MRC OX90] to CD200 / OX2 - BSA and Azide free
  • Host species

    Rat
  • Tested applications

    Suitable for: IHC-Fr, IHC-FoFr, Flow Cyt, ELISAmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Fusion protein corresponding to CD200/ OX2. Mouse CD200-rat CD4 fusion protein.

  • Positive control

    • FC: C57BL/6 splenocytes.
  • General notes

    ab244564 is a PBS-only buffer format of ab33734. Please refer to ab33734 for recommended dilutions, protocols, and image data.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. 

    For details on our patents, please refer to www.abcam.com/RabMAbPatents

    Spleen cells from immunised rats were fused with cells of the rat Y3 myeloma cell line.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

Properties

Applications

Our Abpromise guarantee covers the use of ab244564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.
Flow Cyt Use a concentration of 0.2 µg/ml.
ELISA Use at an assay dependent concentration.

Target

  • Function

    Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues.
  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Antigen identified by monoclonal antibody MRC OX 2 antibody
    • CD200 antibody
    • CD200 antigen antibody
    • CD200 molecule antibody
    • MOX1 antibody
    • MOX2 antibody
    • MRC antibody
    • MRC OX 2 antigen antibody
    • My033 antibody
    • OX 2 antibody
    • OX 2 membrane glycoprotein precursor antibody
    • OX-2 membrane glycoprotein antibody
    • OX2G antibody
    • OX2G_HUMAN antibody
    see all

Images

  • This data was developed using the same antibody clone in a different buffer formulation (ab33734)

    C57BL/6 mouse splenocytes stained with ab33734 (right) or Rat IgG2aκ (ab18450) isotype (left). C57BL/6 mouse splenocytes were incubated for 30 min on ice in 1x PBS / 10 % mouse serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab33734) or Rat IgG2aκ (ab18450) (1x106 in 100 µl at 0.2 µg/ml) for 30 min on ice.

    The secondary antibody Goat anti-rat IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150165) was used at 1/2000 dilution for 30 min on ice. The cells were simultaneously stained with CD19 antibody.

    Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.

     

References

ab244564 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab244564.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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