Overview

  • Product name

    Anti-CD200 / OX2 antibody [OX-104]
    See all CD200 / OX2 primary antibodies
  • Description

    Mouse monoclonal [OX-104] to CD200 / OX2
  • Host species

    Mouse
  • Specificity

    ab23552 recognises CD200 cell surface antigen expressed by a subset of B lymphocytes, some endothelial cells and by neurons.

  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Fusion protein corresponding to Human CD200/ OX2 (extracellular). Recombinant fusion protein consisting of the extracellular region of human CD200/ OX2 fused with domains three and four of rat CD4. Full details of the immunogen can be found in PubMed ID 11260322.
    Database link: P41217

  • Positive control

    • Flow Cyt: Human peripheral blood mononuclear cells.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab23552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues.
  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Antigen identified by monoclonal antibody MRC OX 2 antibody
    • CD200 antibody
    • CD200 antigen antibody
    • CD200 molecule antibody
    • MOX1 antibody
    • MOX2 antibody
    • MRC antibody
    • MRC OX 2 antigen antibody
    • My033 antibody
    • OX 2 antibody
    • OX 2 membrane glycoprotein precursor antibody
    • OX-2 membrane glycoprotein antibody
    • OX2G antibody
    • OX2G_HUMAN antibody
    see all

Images

  • Human peripheral blood mononuclear cells stained with ab23552 (right) or mouse IgG1 kappa (ab170190) isotype (left). Human peripheral blood mononuclear cells were incubated for 30 min on ice in  1x PBS containing 10 µg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab23552) or mouse IgG1 kappa (ab170190) (1x106 in 100 µl at 1 µg/ml) for 30 min on ice. The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150177) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD19 APC antibody.

    Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.

References

This product has been referenced in:

  • Adhikary G  et al. Identification of a population of epidermal squamous cell carcinoma cells with enhanced potential for tumor formation. PLoS One 8:e84324 (2013). Human . Read more (PubMed: 24376802) »
  • Rasmussen JG  et al. Activation of Protease-Activated Receptor 2 Induces VEGF Independently of HIF-1. PLoS One 7:e46087 (2012). Flow Cyt ; Human . Read more (PubMed: 23049945) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated
Sample
Human Tissue sections (Bone marrow)
Specification
Bone marrow
Permeabilization
No
Fixative
Zinc formalin

Abcam user community

Verified customer

Submitted May 08 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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