Overview

  • Product name
    Anti-CD200 / OX2 antibody [MRC OX90]
    See all CD200 / OX2 primary antibodies
  • Description
    Rat monoclonal [MRC OX90] to CD200 / OX2
  • Host species
    Rat
  • Tested applications
    Suitable for: IHC-FoFr, Flow Cyt, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse CD200-rat CD4 fusion protein.

  • General notes


    Spleen cells from immunised rats were fused with cells of the rat Y3 myeloma cell line.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This purified antibody was prepared by affinity chromatography on Protein G from tissue culture supernatant.
  • Primary antibody notes
    Spleen cells from immunised rats were fused with cells of the rat Y3 myeloma cell line.
  • Clonality
    Monoclonal
  • Clone number
    MRC OX90
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration.
Flow Cyt Use 10µl for 106 cells.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function
    Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues.
  • Sequence similarities
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen identified by monoclonal antibody MRC OX 2 antibody
    • CD200 antibody
    • CD200 antigen antibody
    • CD200 molecule antibody
    • MOX1 antibody
    • MOX2 antibody
    • MRC antibody
    • MRC OX 2 antigen antibody
    • My033 antibody
    • OX 2 antibody
    • OX 2 membrane glycoprotein precursor antibody
    • OX-2 membrane glycoprotein antibody
    • OX2G antibody
    • OX2G_HUMAN antibody
    see all

Images

  • Immunohistochemical analysis of murine brain tissue, staining CD200 / OX2 with ab33734.

    Tissue was fixed with paraformaldehyde and blocked with 5% BSA + 5% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/600 in 5% BSA + 5% NGS) for 17 hours at 4°C. An AlexaFluor®555-conjugated goat anti-rat polyclonal IgG (1/200) was used as the secondary antibody.

    See Abreview

References

ab33734 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (brain)
Specification
brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
BSA + serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Nov 22 2012

Answer

DISCOUNT CODE: 40721
Expiration date: 20. November 2012
I am very pleased to hear your customer would like to accept our offer and test ab33734 in IHC-FoFr.
This code will give your customer the value of ab33734 off a future order before the expiration date.
To redeem this offer, please submit an Abreview for IHC-FoFr and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests.
The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.
Any feedback that your customer can provide will be greatly appreciated, whether positive or negative. If you or your customer has any further questions, please do not hesitate to contact us. We look forward to receiving your customer's Abreview and wish your customer luck with their research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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