Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21232] to CD200R
- Suitable for: IHC-P
- Reacts with: Human
Product nameAnti-CD200R antibody [EPR21232]
See all CD200R primary antibodies
DescriptionRabbit monoclonal [EPR21232] to CD200R
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Database link: Q8TD46
- IHC-P: FFPE human tonsil tissue sections. Human skeletal muscle was used as a negative control tissue.
This product was made using synthetic libraries and phage display technology.
This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).
Please note, not recommended for long term 4°C storage. A long term 4c storage could lead to a higher background signal.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 49% Glycerol, PBS
Concentration information loading...
PurityProtein A purified
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
- Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Our Abpromise guarantee covers the use of ab232941 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
It is recommended to perform standard protocol F with the peroxidase block step moved in between the polymer and DAB incubation on a Leica BONDTM system. Using standard protocol F on Leica BONDTM system is likely to cause higher background with ab232941.
FunctionReceptor for the CD200/OX2 cell surface glycoprotein. Also binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200.
Tissue specificityExpressed in granulocytes, monocytes, most T cells, neutrophils, basophils and a subset of NK, NKT and B cells (at protein level). Expressed in bone marrow, lymph nodes, spleen, lung, liver, spinal cord, kidney. Expressed in monocyte-derived dendritic and mast cells.
Sequence similaritiesBelongs to the CD200R family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsThe mature form of isoform 2 and/or isoform 4 starts at sequence position 27 of the corresponding isoform.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- CD 200R antibody
- CD200 cell surface glycoprotein receptor antibody
- CD200 receptor 1 antibody
IHC image of CD200R1 staining in a section of formalin-fixed paraffin-embedded normal human tonsil and human normal skeletal muscle* performed on a Leica BONDTM system using the standard protocol F with the peroxidase block step moved in between the polymer and DAB incubation. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab232941 at 0.1µg/ml for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232941 has not yet been referenced specifically in any publications.