Recombinant

Recombinant Anti-CD200R antibody [EPR21232] (ab232941)

Overview

  • Product name

    Anti-CD200R antibody [EPR21232]
    See all CD200R primary antibodies
  • Description

    Rabbit monoclonal [EPR21232] to CD200R
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment.
    Database link: Q8TD46

  • Positive control

    • IHC-P: FFPE human tonsil tissue sections. Human skeletal muscle was used as a negative control tissue.
  • General notes

    This product was made using synthetic libraries and phage display technology.

    This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).

    Please note, not recommended for long term 4°C storage. A long term 4c storage could lead to a higher background signal.

Properties

Applications

Our Abpromise guarantee covers the use of ab232941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

 

It is recommended to perform standard protocol F with the peroxidase block step moved in between the polymer and DAB incubation on a Leica BONDTM system. Using standard protocol F on Leica BONDTM system is likely to cause higher background with ab232941. 

Target

  • Function

    Receptor for the CD200/OX2 cell surface glycoprotein. Also binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200.
  • Tissue specificity

    Expressed in granulocytes, monocytes, most T cells, neutrophils, basophils and a subset of NK, NKT and B cells (at protein level). Expressed in bone marrow, lymph nodes, spleen, lung, liver, spinal cord, kidney. Expressed in monocyte-derived dendritic and mast cells.
  • Sequence similarities

    Belongs to the CD200R family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    The mature form of isoform 2 and/or isoform 4 starts at sequence position 27 of the corresponding isoform.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 200R antibody
    • CD200 cell surface glycoprotein receptor antibody
    • CD200 receptor 1 antibody
    • Cd200r1 antibody
    • Cell surface glycoprotein CD200 receptor 1 antibody
    • Cell surface glycoprotein OX2 receptor 1 antibody
    • Cell surface glycoprotein OX2 receptor antibody
    • cell surface glycoprotein receptor CD200 antibody
    • CRTR 2 antibody
    • CRTR2 antibody
    • HCRTR2 antibody
    • MO2R1_HUMAN antibody
    • MOX2 receptor antibody
    • MOX2R antibody
    • OX2R antibody
    see all

Images

  • IHC image of CD200R1 staining in a section of formalin-fixed paraffin-embedded normal human tonsil and human normal skeletal muscle* performed on a Leica BONDTM system using the standard protocol F with the peroxidase block step moved in between the polymer and DAB incubation. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab232941 at 0.1µg/ml for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

     

References

ab232941 has not yet been referenced specifically in any publications.

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