Anti-CD200R antibody [OX102] (ab243838)
Key features and details
- Mouse monoclonal [OX102] to CD200R
- Suitable for: IHC-Fr, Flow Cyt
- Reacts with: Rat
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-CD200R antibody [OX102]
See all CD200R primary antibodies -
Description
Mouse monoclonal [OX102] to CD200R -
Host species
Mouse -
Tested applications
Suitable for: IHC-Fr, Flow Cytmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Tissue, cells or virus corresponding to Rat CD200R. Rat peritoneal exudate cells.
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Positive control
- IHC-Fr: Rat spleen tissue. Flow Cyt: Lewis rat splenocytes.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
OX102 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab243838 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use a concentration of 5 µg/ml.
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Flow Cyt |
Use at an assay dependent concentration.
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Notes |
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IHC-Fr
Use a concentration of 5 µg/ml. |
Flow Cyt
Use at an assay dependent concentration. |
Target
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Function
Receptor for the CD200/OX2 cell surface glycoprotein. Also binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200. -
Tissue specificity
Expressed in granulocytes, monocytes, most T cells, neutrophils, basophils and a subset of NK, NKT and B cells (at protein level). Expressed in bone marrow, lymph nodes, spleen, lung, liver, spinal cord, kidney. Expressed in monocyte-derived dendritic and mast cells. -
Sequence similarities
Belongs to the CD200R family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsThe mature form of isoform 2 and/or isoform 4 starts at sequence position 27 of the corresponding isoform. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 64357 Rat
- SwissProt: Q9ES58 Rat
- Unigene: 124439 Rat
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Alternative names
- CD 200R antibody
- CD200 cell surface glycoprotein receptor antibody
- CD200 receptor 1 antibody
see all
Images
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ab170190) isotype (left). Cells were incubated for 30 min on ice in 1x PBS containing 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab243838) or mouse IgG1κ (ab170190) isotype (1x 10ab150117) was used at 1/2000 dilution for 30 min on ice.
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IHC image of CD200R staining in a section of frozen normal rat spleen*.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab243838 (shown in green) at 5μg/ml and co-localisation of T cells stained using ab16669 (shown in red), Rabbit monoclonal to CD3, at 1/150 dilution. The section was then incubated with ab150117 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 488, 1/1000)) presabsorbed and ab150080 (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594, 1/1000)) for 1 hour at room temperature. The secondary-only control image is taken from an identical assay without primary antibody. The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.
*Tissue obtained from Charles River.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab243838 has been referenced in 1 publication.
- Wright GJ et al. Lymphoid/neuronal cell surface OX2 glycoprotein recognizes a novel receptor on macrophages implicated in the control of their function. Immunity 13:233-42 (2000). PubMed: 10981966