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    cd204-antibody-ab217843.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-CD204 antibody (ab217843)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD204 antibody (ab217843)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD204 antibody (ab217843)
  • Western blot - Anti-CD204 antibody (ab217843)

Key features and details

  • Rabbit polyclonal to CD204
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-CD204 antibody
    See all CD204 primary antibodies
  • Description

    Rabbit polyclonal to CD204
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Rat
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human CD204 aa 200-300 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P21757

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human glioma and esophageal cancer tissues. Human colon carcinoma lysates.
  • General notes

     This product was previously labelled as Macrophage Scavenger Receptor I

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Innate and adaptive immunity
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipoprotein metabolism
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Related Products

    • Recombinant Human CD204 protein (denatured) (ab203525)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab217843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Rat
Human
All applications
Mouse
Rat
Application Abreviews Notes
WB
1/100 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/100 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Membrane glycoproteins implicated in the pathologic deposition of cholesterol in arterial walls during atherogenesis. Two types of receptor subunits exist. These receptors mediate the endocytosis of a diverse group of macromolecules, including modified low density lipoproteins (LDL). Isoform III does not internalize actetylated LDL.
  • Tissue specificity

    Isoform I, isoform II and isoform III are expressed in monocyte-derived macrophages.
  • Sequence similarities

    Contains 1 collagen-like domain.
    Contains 1 SRCR domain.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P21757 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4481 Human
    • Entrez Gene: 20288 Mouse
    • Entrez Gene: 498638 Rat
    • Omim: 153622 Human
    • SwissProt: P21757 Human
    • SwissProt: P30204 Mouse
    • Unigene: 147635 Human
    • Unigene: 239291 Mouse
    • Unigene: 231068 Rat
    see all
  • Alternative names

    • CD204 antibody
    • CD204 antigen antibody
    • CD24 antibody
    • Macrophage acetylated LDL receptor I and II antibody
    • Macrophage scavenger receptor 1 antibody
    • Macrophage scavenger receptor type III antibody
    • Macrophage scavenger receptor types I and II antibody
    • Msr 1 antibody
    • MSR1 antibody
    • MSRE_HUMAN antibody
    • phSR1 antibody
    • phSR2 antibody
    • SCARA 1 antibody
    • SCARA1 antibody
    • Scavenger receptor class A member 1 antibody
    • Scavenger receptor class A, member 1 antibody
    • Scavenger receptor type A antibody
    • Scvr antibody
    • SR A antibody
    • SRA antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD204 antibody (ab217843)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD204 antibody (ab217843)

    Immunohistochemical analysis of formalin-fixed paraffin-embedded Human glioma tissue, labeling CD204 using ab217843 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD204 antibody (ab217843)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD204 antibody (ab217843)

    Immunohistochemical analysis of formalin-fixed paraffin-embedded Human esophageal cancer tissue, labeling CD204 using ab217843 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

  • Western blot - Anti-CD204 antibody (ab217843)
    Western blot - Anti-CD204 antibody (ab217843)
    All lanes : Anti-CD204 antibody (ab217843) at 1/200 dilution

    Lane 1 : Human colon carcinoma lysates
    Lane 2 : rat brain lysates

    Secondary
    All lanes : goat anti-rabbit IgG antibody (H+L) HRP-conjugated at 1/3000 dilution

    Developed using the ECL technique.

    Predicted band size: 41 kDa
    Observed band size: 41 kDa



    12% gel

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab217843? Please let us know so that we can cite the reference in this datasheet.

    ab217843 has not yet been referenced specifically in any publications.

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