Anti-CD204 antibody (ab217843)
Key features and details
- Rabbit polyclonal to CD204
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CD204 antibody
See all CD204 primary antibodies -
Description
Rabbit polyclonal to CD204 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB RatHuman
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Immunogen
Synthetic peptide within Human CD204 aa 200-300 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P21757 -
Positive control
- Human glioma and esophageal cancer tissues. Human colon carcinoma lysates.
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General notes
This product was previously labelled as Macrophage Scavenger Receptor I
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab217843 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Rat
Human
|
| All applications |
Mouse
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/100 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
|
|
| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/100 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). |
|
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Membrane glycoproteins implicated in the pathologic deposition of cholesterol in arterial walls during atherogenesis. Two types of receptor subunits exist. These receptors mediate the endocytosis of a diverse group of macromolecules, including modified low density lipoproteins (LDL). Isoform III does not internalize actetylated LDL. -
Tissue specificity
Isoform I, isoform II and isoform III are expressed in monocyte-derived macrophages. -
Sequence similarities
Contains 1 collagen-like domain.
Contains 1 SRCR domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4481 Human
- Entrez Gene: 20288 Mouse
- Entrez Gene: 498638 Rat
- Omim: 153622 Human
- SwissProt: P21757 Human
- SwissProt: P30204 Mouse
- Unigene: 147635 Human
- Unigene: 239291 Mouse
see all -
Alternative names
- CD204 antibody
- CD204 antigen antibody
- CD24 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD204 antibody (ab217843)
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human glioma tissue, labeling CD204 using ab217843 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD204 antibody (ab217843)Immunohistochemical analysis of formalin-fixed paraffin-embedded Human esophageal cancer tissue, labeling CD204 using ab217843 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Western blot - Anti-CD204 antibody (ab217843)All lanes : Anti-CD204 antibody (ab217843) at 1/200 dilution
Lane 1 : Human colon carcinoma lysates
Lane 2 : rat brain lysates
Secondary
All lanes : goat anti-rabbit IgG antibody (H+L) HRP-conjugated at 1/3000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa
Observed band size: 41 kDa12% gel
Protocols
Datasheets and documents
References (0)
ab217843 has not yet been referenced specifically in any publications.
