Anti-CD204 antibody (ab217843)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CD204 antibody
See all CD204 primary antibodies -
Description
Rabbit polyclonal to CD204 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human CD204 aa 230-272 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Sequence:KEEQVHLEQEIKGEVKVLNNITNDLRLKDWEHSQTLRNITLIQ
Database link: P21757 -
Positive control
- Human glioma and esophageal cancer tissues. Human colon carcinoma lysates.
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General notes
This product was previously labelled as Macrophage Scavenger Receptor I
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab217843 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/100 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). | |
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Membrane glycoproteins implicated in the pathologic deposition of cholesterol in arterial walls during atherogenesis. Two types of receptor subunits exist. These receptors mediate the endocytosis of a diverse group of macromolecules, including modified low density lipoproteins (LDL). Isoform III does not internalize actetylated LDL. -
Tissue specificity
Isoform I, isoform II and isoform III are expressed in monocyte-derived macrophages. -
Sequence similarities
Contains 1 collagen-like domain.
Contains 1 SRCR domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4481 Human
- Entrez Gene: 20288 Mouse
- Entrez Gene: 498638 Rat
- Omim: 153622 Human
- SwissProt: P21757 Human
- SwissProt: P30204 Mouse
- Unigene: 147635 Human
- Unigene: 239291 Mouse
see all -
Alternative names
- CD204 antibody
- CD204 antigen antibody
- CD24 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD204 antibody (ab217843)Immunohistochemical analysis of formalin-fixed paraffin-embedded Human glioma tissue, labeling CD204 using ab217843 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD204 antibody (ab217843)Immunohistochemical analysis of formalin-fixed paraffin-embedded Human esophageal cancer tissue, labeling CD204 using ab217843 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Western blot - Anti-CD204 antibody (ab217843)All lanes : Anti-CD204 antibody (ab217843) at 1/200 dilution
Lane 1 : Human colon carcinoma lysates
Lane 2 : rat brain lysates
Secondary
All lanes : goat anti-rabbit IgG antibody (H+L) HRP-conjugated at 1/3000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa
Observed band size: 41 kDa12% gel
Datasheets and documents
References
ab217843 has not yet been referenced specifically in any publications.
