Recombinant Anti-CD204 antibody [EPR7536] (ab151707)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7536] to CD204
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CD204 antibody [EPR7536]
See all CD204 primary antibodies -
Description
Rabbit monoclonal [EPR7536] to CD204 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human CD204 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P21757 -
Positive control
- WB: Human placenta, THP1 and HeLa lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7536 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151707 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 50 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 50 kDa. |
Target
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Function
Membrane glycoproteins implicated in the pathologic deposition of cholesterol in arterial walls during atherogenesis. Two types of receptor subunits exist. These receptors mediate the endocytosis of a diverse group of macromolecules, including modified low density lipoproteins (LDL). Isoform III does not internalize actetylated LDL. -
Tissue specificity
Isoform I, isoform II and isoform III are expressed in monocyte-derived macrophages. -
Sequence similarities
Contains 1 collagen-like domain.
Contains 1 SRCR domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4481 Human
- Entrez Gene: 20288 Mouse
- Entrez Gene: 498638 Rat
- Omim: 153622 Human
- SwissProt: P21757 Human
- SwissProt: P30204 Mouse
- Unigene: 147635 Human
- Unigene: 239291 Mouse
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Alternative names
- CD204 antibody
- CD204 antigen antibody
- CD24 antibody
see all
Images
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Anti-CD204 antibody [EPR7536] (ab151707) at 1/5000 dilution (purified) + Rat placenta tissue lysate at 20 µg
Secondary
HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
Anti-CD204 antibody [EPR7536] (ab151707) at 1/1000 dilution (purified) + Mouse placenta tissue lysate at 20 µg
Secondary
HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
All lanes : Anti-CD204 antibody [EPR7536] (ab151707) at 1/1000 dilution (purified)
Lane 1 : THP-1 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
All lanes : Anti-CD204 antibody [EPR7536] (ab151707) at 1/1000 dilution
Lane 1 : Human placenta tissue lysate
Lane 2 : THP1 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 50 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab151707 has been referenced in 8 publications.
- Jia J et al. Ferritin triggers neutrophil extracellular trap-mediated cytokine storm through Msr1 contributing to adult-onset Still's disease pathogenesis. Nat Commun 13:6804 (2022). PubMed: 36357401
- Gwon MH et al. Phenethyl Isothiocyanate Protects against High Fat/Cholesterol Diet-Induced Obesity and Atherosclerosis in C57BL/6 Mice. Nutrients 12:N/A (2020). PubMed: 33261070
- Lv JJ et al. Blockade of Macrophage CD147 Protects Against Foam Cell Formation in Atherosclerosis. Front Cell Dev Biol 8:609090 (2020). PubMed: 33490072
- Guo M et al. Triggering MSR1 promotes JNK-mediated inflammation in IL-4-activated macrophages. EMBO J 38:N/A (2019). PubMed: 31028084
- Lindsey ML et al. Exogenous CXCL4 infusion inhibits macrophage phagocytosis by limiting CD36 signalling to enhance post-myocardial infarction cardiac dilation and mortality. Cardiovasc Res 115:395-408 (2019). PubMed: 30169632
- Wang C et al. CTRP13 inhibits atherosclerosis via autophagy-lysosome-dependent degradation of CD36. FASEB J 33:2290-2300 (2019). PubMed: 30222079
- Ding Z et al. PCSK9 regulates expression of scavenger receptors and ox-LDL uptake in macrophages. Cardiovasc Res 114:1145-1153 (2018). PubMed: 29617722
- Li P et al. Scavenger receptor-mediated Ad5 entry and acLDL accumulation in monocytes/macrophages synergistically trigger innate responses against viral infection. Virology 519:86-98 (2018). PubMed: 29680370