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    cd208-antibody-ab153932.pdf

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Tags & Cell Markers Subcellular Markers Organelles Lysosome
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Anti-CD208 antibody (ab153932)

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Immunocytochemistry/ Immunofluorescence - Anti-CD208 antibody (ab153932)
  • Western blot - Anti-CD208 antibody (ab153932)

Key features and details

  • Rabbit polyclonal to CD208
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-CD208 antibody
    See all CD208 primary antibodies
  • Description

    Rabbit polyclonal to CD208
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Monkey
  • Immunogen

    Recombinant fragment corresponding to Human CD208 aa 116-372.

  • Positive control

    • HeLa cells; HeLa and MOLT4 whole cell lysates.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Lysosome
    • Cancer
    • Tumor biomarkers
    • Other
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Dendritic Cell Lineage

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • MOLT-4 whole cell lysate (ab7912)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab153932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 44 kDa.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 44 kDa.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Might change the lysosome function after the transfer of peptide-MHC class II molecules to the surface of dendritic cells.
  • Tissue specificity

    Expressed in lymphoid organs and dendritic cells. Expressed in lung. Up-regulated in carcinomas of the esophagus, colon, rectum, ureter, stomach, breast, fallopian tube, thyroid and parotid tissues.
  • Sequence similarities

    Belongs to the LAMP family.
  • Cellular localization

    Lysosome membrane.
  • Target information above from: UniProt accession Q9UQV4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 27074 Human
    • Omim: 605883 Human
    • SwissProt: Q9UQV4 Human
    • Unigene: 518448 Human
    • Alternative names

      • CD208 antibody
      • CD208 antigen antibody
      • DC LAMP antibody
      • DC lysosome associated membrane glycoprotein antibody
      • DC-lysosome-associated membrane glycoprotein antibody
      • DCLAMP antibody
      • LAMP antibody
      • LAMP 3 antibody
      • LAMP-3 antibody
      • Lamp3 antibody
      • LAMP3_HUMAN antibody
      • Lysosomal associated membrane protein 3 antibody
      • Lysosomal-associated membrane protein 3 antibody
      • Lysosome-associated membrane glycoprotein 3 antibody
      • Protein TSC403 antibody
      • TSC403 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-CD208 antibody (ab153932)
      Immunocytochemistry/ Immunofluorescence - Anti-CD208 antibody (ab153932)

      Immunofluorescence analysis of methanol-fixed HeLa cells, labeling CD208 with ab153932 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.

    • Western blot - Anti-CD208 antibody (ab153932)
      Western blot - Anti-CD208 antibody (ab153932)
      Anti-CD208 antibody (ab153932) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

      Predicted band size: 44 kDa



      10% SDS PAGE

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab153932? Please let us know so that we can cite the reference in this datasheet.

    ab153932 has not yet been referenced specifically in any publications.

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