Recombinant
RabMAb

Recombinant Anti-CD21 antibody [EP3093] (HRP) (ab202354)

Overview

  • Product name

    Anti-CD21 antibody [EP3093] (HRP)
    See all CD21 primary antibodies
  • Description

    Rabbit monoclonal [EP3093] to CD21 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human CD21 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Raji whole cell lysate. IHC-P: FFPE human tonsil (normal) tissue sections.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Proclin
    Constituents: PBS, 30% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP3093
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab202354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 155 kDa (predicted molecular weight: 113 kDa).
IHC-P 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Receptor for complement C3Dd, for the Epstein-Barr virus on human B-cells and T-cells and for HNRPU. Participates in B lymphocytes activation.
  • Tissue specificity

    Mature B-lymphocytes, T-lymphocytes, pharyngeal epithelial cells, astrocytes and follicular dendritic cells of the spleen.
  • Involvement in disease

    Genetic variations in CR2 are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9) [MIM:610927]. Systemic lupus erythematosus (SLE) is a chronic autoimmune disease with a complex genetic basis. SLE is an inflammatory, and often febrile multisystemic disorder of connective tissue characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similarities

    Belongs to the receptors of complement activation (RCA) family.
    Contains 15 Sushi (CCP/SCR) domains.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • C3DR antibody
    • CD 21 antibody
    • CD21 antibody
    • Complement C3d receptor 2 antibody
    • Complement C3d receptor antibody
    • Complement component (3d/Epstein Barr virus) receptor 2 antibody
    • Complement receptor type 2 antibody
    • CR antibody
    • Cr2 antibody
    • CR2_HUMAN antibody
    • CVID7 antibody
    • EBV receptor antibody
    • EBV-R antibody
    • Epstein Barr virus receptor antibody
    • Epstein-Barr virus receptor antibody
    • EVBR antibody
    • SLEB9 antibody
    see all

Images

  • IHC image of CD21 staining in a section of formalin-fixed paraffin-embedded normal human tonsil tissue*, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab202354, 1/250 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Anti-CD21 antibody [EP3093] (HRP) (ab202354) at 1/5000 dilution + Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 113 kDa
    Observed band size: 155 kDa
    why is the actual band size different from the predicted?


    Exposure time: 8 minutes


    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab202354 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab202354 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab202354.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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