Recombinant Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)
![Western blot - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)](https://www.abcam.com/ps/products/193/ab193554/Images/ab193554-478769-anti-cd21-antibody-ep3093-westernblot-human.jpg "Western blot - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP3093] to CD21 - Low endotoxin, Azide free
- Suitable for: ICC/IF, IP, IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free
See all CD21 primary antibodies -
Description
Rabbit monoclonal [EP3093] to CD21 - Low endotoxin, Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IP: Daudi whole cell lysate.
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General notes
ab193554 is the carrier-free version of ab75985.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP3093 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab193554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| WB |
Use at an assay dependent concentration.
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| Notes |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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WB
Use at an assay dependent concentration. |
Target
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Function
Receptor for complement C3Dd, for the Epstein-Barr virus on human B-cells and T-cells and for HNRPU. Participates in B lymphocytes activation. -
Tissue specificity
Mature B-lymphocytes, T-lymphocytes, pharyngeal epithelial cells, astrocytes and follicular dendritic cells of the spleen. -
Involvement in disease
Genetic variations in CR2 are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9) [MIM:610927]. Systemic lupus erythematosus (SLE) is a chronic autoimmune disease with a complex genetic basis. SLE is an inflammatory, and often febrile multisystemic disorder of connective tissue characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
Sequence similarities
Belongs to the receptors of complement activation (RCA) family.
Contains 15 Sushi (CCP/SCR) domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 1380 Human
- Omim: 120650 Human
- SwissProt: P20023 Human
- Unigene: 445757 Human
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Alternative names
- C3DR antibody
- CD 21 antibody
- CD21 antibody
see all
Images
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Western blot - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)All lanes : Anti-CD21 antibody [EP3093] (ab75985) at 1/20000 dilution (Purified)
Lane 1 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate
Lane 2 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution -
![Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)](https://www.abcam.com/ps/products/193/ab193554/Images/ab193554-14-anti-cd21-antibody-ep3093-immunocytochemistry-raji-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)This data was developed using ab193554, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of Raji(Human Burkitt's lymphoma B lymphocyte) cells labeling CD21 with Purified ab193554 at 1:50 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A]+H21:L21 - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)](https://www.abcam.com/ps/products/193/ab193554/Images/ab193554-13-anti-cd21-antibody-ep3093-immunohistochemistry-tonsil-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)This data was developed using ab75985, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling CD21 with Purified ab75985 at 1:8000 dilution (0.066 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. -
![Immunoprecipitation - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)](https://www.abcam.com/ps/products/193/ab193554/Images/ab193554-9-anti-cd21-antibody-ep3093-immunoprecipitation-daudi-human.jpg)
Immunoprecipitation - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)Purified ab75985 at 1/100 dilution (2µg) immunoprecipitating CD21 in Daudi whole cell lysate.
Lane 1 (input): Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate 10µg
Lane 2 (+): ab75985 + Daudi whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab75985 in Daudi whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 155 kDa
The band at around 15kDa could be cleavage form. (PMID: 9647256)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75985). -
![Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)](https://www.abcam.com/ps/products/193/ab193554/Images/ab193554-10-benefits-of-recombinant-antibodies.png)
Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab193554 has not yet been referenced specifically in any publications.
