Recombinant
RabMAb

Recombinant Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP3093] to CD21 - Low endotoxin, Azide free
  • Suitable for: ICC/IF, IP, IHC-P, Flow Cyt
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free
    See all CD21 primary antibodies

  • Description

    Rabbit monoclonal [EP3093] to CD21 - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Does not react with: Rat

  • Immunogen

    Synthetic peptide within Human CD21 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Daudi cell lysate, Raji cell lysate and Tonsil tissue IP: Daudi whole cell lysate.

  • General notes

    ab193554 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EP3093

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab193554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Receptor for complement C3Dd, for the Epstein-Barr virus on human B-cells and T-cells and for HNRPU. Participates in B lymphocytes activation.

  • Tissue specificity

    Mature B-lymphocytes, T-lymphocytes, pharyngeal epithelial cells, astrocytes and follicular dendritic cells of the spleen.

  • Involvement in disease

    Genetic variations in CR2 are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9) [MIM:610927]. Systemic lupus erythematosus (SLE) is a chronic autoimmune disease with a complex genetic basis. SLE is an inflammatory, and often febrile multisystemic disorder of connective tissue characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.

  • Sequence similarities

    Belongs to the receptors of complement activation (RCA) family.
    Contains 15 Sushi (CCP/SCR) domains.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • C3DR antibody
      • CD 21 antibody
      • CD21 antibody
      • Complement C3d receptor 2 antibody
      • Complement C3d receptor antibody
      • Complement component (3d/Epstein Barr virus) receptor 2 antibody
      • Complement receptor type 2 antibody
      • CR antibody
      • Cr2 antibody
      • CR2_HUMAN antibody
      • CVID7 antibody
      • EBV receptor antibody
      • EBV-R antibody
      • Epstein Barr virus receptor antibody
      • Epstein-Barr virus receptor antibody
      • EVBR antibody
      • SLEB9 antibody
      see all

    Images

    • Flow Cytometry - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)
      Flow Cytometry - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)This image is courtesy of an anonymous Abreview.
      ab75985 staining CD21 in Cynomolgus Monkey whole blood by Flow Cytometry. Red blood cells were lysed in PBS + 1% BSA and 0.01% sodium azide, fixed in paraformaldehyde and incubated with the primary antibody (1/100 in PBS + 1% BSA and 0.01% sodium azide) for 1 hour at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.
      Gating Strategy: Live Lymphocytes.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75985).

    • Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)
      Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)

      ab75985 staining CD21 antibody in Raji (human Burkitt's lymphoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000.

      DAPI was used as a nuclear counterstain and the negative control was PBS only.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75985).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)

      ab75985 showing positive staining in Normal tonsil tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75985).

    • Immunoprecipitation - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)
      Immunoprecipitation - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)

      Purified ab75985 at 1/100 dilution (2µg) immunoprecipitating CD21 in Daudi whole cell lysate.
      Lane 1 (input): Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate 10µg
      Lane 2 (+): ab75985 + Daudi whole cell lysate.
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab75985 in Daudi whole cell lysate.
      VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
      Blocking Buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM/TBST.
      Observed band size: 155 kDa
      The band at around 15kDa could be cleavage form. (PMID: 9647256)
      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75985).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)

      ab75985 showing negative staining in Normal liver tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75985).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)

      ab75985 showing negative staining in Normal kidney tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75985).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)

      ab75985 showing negative staining in Normal breast tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75985).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)

      This IHC data was generated using the same anti-CD21 antibody clone, EP3093, in a different buffer formulation (cat# ab75985).

      ab75985 showing positive staining in Normal tonsil tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)
      Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)

      This ICC/IF data was generated using the same anti-CD21 antibody clone, EP3093, in a different buffer formulation (cat# ab75985).

      ab75985 staining CD21 antibody in Raji (human Burkitt's lymphoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000.

      DAPI was used as a nuclear counterstain and the negative control was PBS only.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References

    Publishing research using ab193554? Please let us know so that we can cite the reference in this datasheet.

    ab193554 has not yet been referenced specifically in any publications.

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