Recombinant
RabMAb

Recombinant Anti-CD21 antibody [SP186] - C-terminal (ab227662)

Overview

  • Product name

    Anti-CD21 antibody [SP186] - C-terminal
    See all CD21 primary antibodies
  • Description

    Rabbit monoclonal [SP186] to CD21 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human CD21 aa 1000 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P20023

  • Positive control

    • WB: Daudi cell lysate. Flow Cyt: Raji and 2.4G2 cells. IHC-P: Human tonsil, reactive lymph node, spleen and colon tissues, rat spleen

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.6
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from TCS by protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    SP186
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227662 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/400. Predicted molecular weight: 113 kDa.
Flow Cyt 1/100.

Target

  • Function

    Receptor for complement C3Dd, for the Epstein-Barr virus on human B-cells and T-cells and for HNRPU. Participates in B lymphocytes activation.
  • Tissue specificity

    Mature B-lymphocytes, T-lymphocytes, pharyngeal epithelial cells, astrocytes and follicular dendritic cells of the spleen.
  • Involvement in disease

    Genetic variations in CR2 are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9) [MIM:610927]. Systemic lupus erythematosus (SLE) is a chronic autoimmune disease with a complex genetic basis. SLE is an inflammatory, and often febrile multisystemic disorder of connective tissue characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similarities

    Belongs to the receptors of complement activation (RCA) family.
    Contains 15 Sushi (CCP/SCR) domains.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • C3DR antibody
    • CD 21 antibody
    • CD21 antibody
    • Complement C3d receptor 2 antibody
    • Complement C3d receptor antibody
    • Complement component (3d/Epstein Barr virus) receptor 2 antibody
    • Complement receptor type 2 antibody
    • CR antibody
    • Cr2 antibody
    • CR2_HUMAN antibody
    • CVID7 antibody
    • EBV receptor antibody
    • EBV-R antibody
    • Epstein Barr virus receptor antibody
    • Epstein-Barr virus receptor antibody
    • EVBR antibody
    • SLEB9 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD21 with ab227662 at 1/100 dilution (1.06 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab227662 for 10 mins at room temperature.

  • Flow Cytometry analysis of Raji (human Burkitt's lymphoma B lymphocyte) cells labeling CD21 with purified ab227662 at 1:20 dilution (5.3 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.
  • Anti-CD21 antibody [SP186] - C-terminal (ab227662) at 1/400 dilution + Daudi (Human Burkitt's lymphoma cell line) cell lysate

    Predicted band size: 113 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling CD21 with ab227662 at 1/100 dilution (1.06 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the rat spleen, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab227662 for 10 mins at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling CD21 with ab227662 at 1/100 dilution (1.06 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human spleen, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab227662 for 10 mins at room temperature.

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD21 using ab227662 at 1/100 dilution in immunohistochemical analysis.

  • Flow Cytometry analysis of 2.4G2 (rat B cell lymphoma B lymphocyte) cells labeling CD21 with purified ab227662 at 1:200 dilution (0.53 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.
  • Flow cytometric analysis of Raji (human Burkitt's lymphoma cell line) cell line labeling CD21 with ab227662 at 1/100 dilution (green) compared with a Rabbit IgG negative control (blue). 

  • Formalin-fixed, paraffin-embedded human reactive lymph node tissue stained for CD21 using ab227662 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human spleen tissue stained for CD21 using ab227662 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human colon tissue stained for CD21 using ab227662 at 1/100 dilution in immunohistochemical analysis.

References

ab227662 has not yet been referenced specifically in any publications.

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